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对拟南芥SUS2在种子中的定位研究揭示了其与质体的紧密关联。

Study of AtSUS2 localization in seeds reveals a strong association with plastids.

作者信息

Núñez Juan Gabriel Angeles, Kronenberger Jocelyne, Wuillème Sylvie, Lepiniec Loïc, Rochat Christine

机构信息

INRA, Seed Biology Laboratory, UMR 204, INRA-AgroParisTech, Institut Jean-Pierre Bourgin, INRA, RD10, 78026 Versailles Cedex, France.

出版信息

Plant Cell Physiol. 2008 Oct;49(10):1621-6. doi: 10.1093/pcp/pcn117. Epub 2008 Aug 12.

Abstract

Sucrose synthase (SUS) is a key enzyme in sucrose metabolism. This enzyme catalyzes the reversible conversion of sucrose and UDP to UDP-glucose and fructose. In the Arabidopsis SUS gene family (six members), SUS2 is strongly and specifically expressed in Arabidopsis seeds during the maturation phase. Using specific antibodies, we have shown that SUS2 is localized in the embryo, endosperm and seed coat with differential patterns. During the maturation phase, the SUS2 protein seems to be mainly co-localized with plastids in the embryo. This novel finding is discussed in relation to the role of this enzyme in storage organs.

摘要

蔗糖合酶(SUS)是蔗糖代谢中的关键酶。该酶催化蔗糖和UDP可逆转化为UDP-葡萄糖和果糖。在拟南芥SUS基因家族(六个成员)中,SUS2在成熟阶段的拟南芥种子中强烈且特异性表达。使用特异性抗体,我们已表明SUS2以不同模式定位于胚、胚乳和种皮中。在成熟阶段,SUS2蛋白似乎主要在胚中与质体共定位。结合该酶在贮藏器官中的作用对这一新发现进行了讨论。

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