全血作为检测丙型肝炎病毒RNA的血浆替代物。
Whole blood as an alternative to plasma for detection of hepatitis C virus RNA.
作者信息
Daniel Hubert Darius J, David Joel, Grant Paul R, Garson Jeremy A, Chandy George M, Abraham Priya
机构信息
Department of Clinical Virology, Christian Medical College, Ida Scudder Road, Vellore 632 004, India.
出版信息
J Clin Microbiol. 2008 Nov;46(11):3791-4. doi: 10.1128/JCM.00045-08. Epub 2008 Aug 13.
Peripheral blood mononuclear cells are reported to be one of the extrahepatic replication sites contributing to the persistence of hepatitis C virus (HCV) infection. Whole-blood and plasma samples from 61 individuals were compared as sources for the detection of HCV RNA. Forty-four of the individuals were receiving antiviral therapy, while 17 were treatment naïve. The quantitation of HCV RNA was done by a sensitive in-house real-time reverse transcription-PCR. When the viral loads in the two types of samples were compared, a correlation coefficient of 0.858 (P < 0.001) was found, indicating that plasma and whole blood are equally acceptable sources for testing for HCV RNA.
据报道，外周血单个核细胞是导致丙型肝炎病毒（HCV）感染持续存在的肝外复制位点之一。对61名个体的全血和血浆样本作为检测HCV RNA的来源进行了比较。其中44名个体正在接受抗病毒治疗，而17名个体未接受过治疗。HCV RNA的定量通过灵敏的内部实时逆转录聚合酶链反应进行。比较两种样本中的病毒载量时，发现相关系数为0.858（P < 0.001），表明血浆和全血是检测HCV RNA同样可接受的来源。
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