Mulligan Elizabeth K, Germer J J, Arens Max Q, D'Amore Krista L, Di Bisceglie Adrian, Ledeboer Nathan A, Moser Michael J, Newman Andrew C, O'Guin Andy K, Olivo Paul D, Podzorski Diane S, Vaughan Kimberly A, Yao Joseph D, Elagin Slava A, Johnson Scott C
EraGen Biosciences, Inc., Madison, WI 53717, USA.
J Clin Microbiol. 2009 Aug;47(8):2635-8. doi: 10.1128/JCM.02170-08. Epub 2009 Jun 17.
A prototype, real-time reverse-transcription PCR assay, based on MultiCode-RTx technology, quantifying hepatitis C virus (HCV) RNA by targeting the HCV 3' untranslated region demonstrated linearity over 7 logs, with a good correlation between the quantitative results of this assay and the results of two commercially available comparator assays for 466 clinical specimens comprising all six HCV genotypes.
一种基于MultiCode-RTx技术的实时逆转录PCR检测原型,通过靶向丙型肝炎病毒(HCV)3'非翻译区对HCV RNA进行定量,在7个对数范围内显示出线性,对于包含所有六种HCV基因型的466份临床标本,该检测的定量结果与两种市售对照检测的结果之间具有良好的相关性。