Erden Ersin Sükrü, Kirkil Gamze, Deveci Figen, Ilhan Nevin, Cobanoğlu Bengü, Turgut Teyfik, Muz Mehmet Hamdi
Erciş State Hospital, Van, Turkey.
Tuberk Toraks. 2008;56(2):127-38.
We aimed to investigate the levels of some chemokines, inflammatory cell counts in bronchoalveolar lavage (BAL) fluid, histopathological changes in lung tissue, to determine the effect of erdosteine on acute inflammatory changes and fibrosis in a rat fibrosis model induced by bleomycine (BLM). Forty-five Wistar male rats were taken into the study. On day 0, intratracheal saline to control group (group 1, n= 15), intratracheal BLM 7.5 U/kg to BLM (group 2, n= 15) and erdosteine group (group 3, n= 15) was administered. In group 3, oral erdosteine (10 mg/kg/day) was applied two days before BLM. On day 0, 14, and 29th five rats in each groups were sacrificed, BAL fluid was performed. Malonyldialdehyde (MDA), macrophage inflammatory protein (MIP)-1alpha, MIP-2 levels in BAL fluid, hydroxyproline levels in lung tissue were measured. Histopathological examination was performed. When BLM group compared to erdosteine group, the levels of MDA, MIP-1alpha, MIP-2, and neutrophil counts, the hydroxyproline (OH-P) level of lung tissue were decreased in erdosteine group on acute inflammatory phase (day 14) (p< 0.001, p= 0.017, p= 0.009, p< 0.001, p= 0.009, respectively), and late fibrosis phase (day 29) except BAL MIP-2 (p= 0.022, p= 0.025, p= 0.01, p< 0.001, respectively). Fibrosis level was significantly lower in erdosteine group than BLM group on day 29 (p= 0.01). We conclude that erdosteine may prevent the acute lung inflammation and fibrosis by suppressing the accumulation of neutrophils, inhibition of lipid peroxydation, chemokine production, and release.
我们旨在研究在博来霉素（BLM）诱导的大鼠纤维化模型中，厄多司坦对急性炎症变化和纤维化的影响，检测支气管肺泡灌洗（BAL）液中某些趋化因子的水平、炎症细胞计数以及肺组织的组织病理学变化。将45只雄性Wistar大鼠纳入研究。第0天，对照组（第1组，n = 15）气管内注入生理盐水，博来霉素组（第2组，n = 15）气管内注入7.5 U/kg的BLM，厄多司坦组（第3组，n = 15）在注入BLM前两天口服厄多司坦（10 mg/kg/天）。在第0、14和29天，每组处死5只大鼠，进行BAL液检测。检测BAL液中丙二醛（MDA）、巨噬细胞炎性蛋白（MIP）-1α、MIP-2水平以及肺组织中羟脯氨酸水平，并进行组织病理学检查。与博来霉素组相比，在急性炎症期（第14天），厄多司坦组的MDA、MIP-1α、MIP-2水平、中性粒细胞计数以及肺组织羟脯氨酸（OH-P）水平均降低（分别为p < 0.001、p = 0.017 p = 0.009、p < 0.001、p = 0.009）；在晚期纤维化期（第29天），除BAL液中MIP-2外（p = 0.022），上述指标也降低（分别为p = 0.025、p = 0.01、p < 0.001）。在第29天，厄多司坦组的纤维化水平显著低于博来霉素组（p = 0.01）。我们得出结论，厄多司坦可能通过抑制中性粒细胞的聚集、脂质过氧化、趋化因子的产生和释放来预防急性肺炎症和纤维化。
