Villordo Sergio M, Gamarnik Andrea V
Fundación Instituto Leloir, Patricias Argentinas 435, Buenos Aires, Argentina.
Virus Res. 2009 Feb;139(2):230-9. doi: 10.1016/j.virusres.2008.07.016. Epub 2008 Sep 9.
Long-range and local RNA-RNA contacts in viral RNA genomes result in tertiary structures that modulate the function of enhancers, promoters, and silencers during translation, RNA replication, and encapsidation. In the case of flaviviruses, the presence of inverted complementary sequences at the 5' and 3' ends of the genome mediate long-range RNA interactions and RNA cyclization. The circular conformation of flavivirus genomes was demonstrated to be essential for RNA amplification. New ideas about the mechanisms by which circular genomes participate in flavivirus replication have emerged in the last few years. Here, we will describe the latest information about cis-acting elements involved in flavivirus genome cyclization, RNA promoter elements required for viral polymerase recognition, and how these elements together coordinate viral RNA synthesis.
病毒RNA基因组中的长程和局部RNA-RNA相互作用会形成三级结构，这些结构在翻译、RNA复制和衣壳化过程中调节增强子、启动子和沉默子的功能。对于黄病毒而言，基因组5'和3'端存在的反向互补序列介导长程RNA相互作用和RNA环化。黄病毒基因组的环状构象已被证明对RNA扩增至关重要。在过去几年中，关于环状基因组参与黄病毒复制机制的新观点不断涌现。在此，我们将描述有关黄病毒基因组环化所涉及的顺式作用元件、病毒聚合酶识别所需的RNA启动子元件的最新信息，以及这些元件如何共同协调病毒RNA合成。
