Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California 94720-7354, USA.
J Virol. 2011 Feb;85(4):1900-5. doi: 10.1128/JVI.02037-10. Epub 2010 Dec 1.
Flaviviruses require complementarity between the 5' and 3' ends of the genome for RNA replication. For mosquito-borne flaviviruses, the cyclization sequences (CS) and upstream of AUG region (UAR) elements at the genomic termini are necessary for viral RNA replication, and a third motif, the downstream of AUG region (DAR), was recently designated for dengue virus. The 3' DAR sequence is also part of a hairpin (HP-3'SL), and both complementarity between 5' and 3' DAR motifs and formation of the HP-3'SL in the absence of the 5' end are conserved among mosquito-borne flaviviruses. Using West Nile virus as a model, we demonstrate that 5'-3' DAR complementarity and HP-3'SL formation are essential for viral RNA replication.
黄病毒的 RNA 复制需要基因组 5' 和 3' 末端之间的互补性。对于蚊媒黄病毒，基因组末端的环化序列 (CS) 和 AUG 区上游 (UAR) 元件是病毒 RNA 复制所必需的，最近还为登革热病毒指定了第三个基序，即 AUG 区下游 (DAR)。3' DAR 序列也是发夹 (HP-3'SL) 的一部分，在没有 5' 末端的情况下，5' 和 3' DAR 基序之间的互补性和 HP-3'SL 的形成在蚊媒黄病毒中是保守的。我们使用西尼罗河病毒作为模型，证明了 5'-3' DAR 互补性和 HP-3'SL 的形成对于病毒 RNA 复制是必不可少的。
