Validation of a quantitative radiographic technique to estimate pulsatile blood flow waveforms using digital subtraction angiographic data.

We have validated a new radiographic technique for determining pulsatile volume flow in arteries following an intraarterial injection of contrast material. Instantaneous blood velocities were estimated by generating a parametric image from dynamic angiographic images in which the image grey level represents contrast material concentration as a function of time and distance along a vessel segment. Adjacent concentration--distance profiles in the parametric image were shifted with respect to distance until a match occurred. A match was defined as the point where the sum of squares of the differences in the two profiles was a minimum. The distance translated per frame interval gives the instantaneous contrast material bolus velocity. We have validated the technique using an experimental phantom of blood circulation, consisting of a pump, flexible plastic tubing, the tubular probe of an electromagnetic flowmeter (EMF) and a solenoid, to simulate a pulsatile flow waveform, which includes reverse flow. Small boluses of contrast material can be injected at various positions in the circuit. Measurements of pulsatile velocity flow were taken at 40 ms intervals, using a tube of 6.6 mm internal diameter and an imaged tube length of 200 mm. The shape of the flow velocity waveform was faithfully reproduced but there was an overestimation of peak velocity of 40% at low velocities (peak velocity of 540 mm s-1), reducing to 19% at peak velocities of 964 mm s-1 with an underestimation of 16% at the peak velocities of 1899 mm s-1. The validation was repeated for distances ranging from 130 to 230 mm between injection and measurement sites and for imaged tube lengths varying from 200 to 20 mm.(ABSTRACT TRUNCATED AT 250 WORDS)