Ustundag Sedat, Yalcin Omer, Sen Saniye, Cukur Ziya, Ciftci Senturk, Demirkan Bora
Trakya University Faculty of Medicine, Department of Nephrology, Edirne, Turkey.
Ren Fail. 2008;30(7):727-35. doi: 10.1080/08860220802212965.
During times of war and natural disasters, rhabdomyolysis-induced myoglobinuric acute renal failure (ARF) can assume epidemic proportions. Free radicals play an important role in the pathogenesis of myoglobinuric ARF. Vitamin C is a major antioxidant, scavenging free radicals. We have not found any studies on the effect of vitamin C on myoglobinuric ARF. Thus, we aimed to investigate the effects of vitamin C on the myoglobinuric ARF formed by glycerol in rats. Three groups of rats were employed in this study. Group 1 served as control, group 2 was given 50% glycerol (10 mL/kg, i.m.), and group 3 was given glycerol plus vitamin C (20 mg/kg, i.p. for four days). Ninety-six hours after glycerol injections, blood samples and kidney tissues were taken from the anesthetized rats. Urea and creatinine levels in plasma; N-acetyl-beta-D-glucosaminidase activity in urine; malondialdehyde levels, superoxide dismutase and catalase enzyme activity in kidney tissue were determined. Histopathological changes and iron accumulation in the kidney tissue were evaluated. In this study, glycerol administration led to marked renal oxidative stress and severe renal functional and morphological deterioration. The treatment of animals with vitamin C partially corrected the renal dysfunction and morphological impairment. In this respect, vitamin C appears to be a promising candidate for the prevention of rhabdomyolysis-induced ARF. Higher dosages of vitamin C than in 20 mg/kg may be beneficial for better functional and morphological recovery in this model ARF.
在战争和自然灾害期间，横纹肌溶解引起的肌红蛋白尿性急性肾衰竭（ARF）可能会呈现流行态势。自由基在肌红蛋白尿性ARF的发病机制中起重要作用。维生素C是一种主要的抗氧化剂，可清除自由基。我们尚未发现任何关于维生素C对肌红蛋白尿性ARF影响的研究。因此，我们旨在研究维生素C对大鼠甘油诱导的肌红蛋白尿性ARF的影响。本研究使用了三组大鼠。第1组作为对照组，第2组给予50%甘油（10 mL/kg，肌肉注射），第3组给予甘油加维生素C（20 mg/kg，腹腔注射，共4天）。甘油注射96小时后，从麻醉的大鼠身上采集血样和肾组织。测定血浆中的尿素和肌酐水平；尿液中的N-乙酰-β-D-氨基葡萄糖苷酶活性；肾组织中的丙二醛水平、超氧化物歧化酶和过氧化氢酶活性。评估肾组织的组织病理学变化和铁蓄积情况。在本研究中，给予甘油导致明显的肾脏氧化应激以及严重的肾功能和形态学恶化。用维生素C治疗动物可部分纠正肾功能障碍和形态学损伤。在这方面，维生素C似乎是预防横纹肌溶解诱导的ARF的一个有前景的候选药物。比20 mg/kg更高剂量的维生素C可能有利于该模型ARF更好的功能和形态学恢复。
