Jiang Enli, He Xiang, Chen Xiancheng, Sun Guojuan, Wu Hongbing, Wei Yuquan, Zhao Xia
Department of Obstetrics and Gynecology, Guizhou Provincial People's Hospital, Guiyang, Guizhou, 550002, People's Republic of China.
Tumori. 2008 May-Jun;94(3):356-61. doi: 10.1177/030089160809400312.
To test the expression level of CD40 on ovarian cancer tissues and its correlation to clinicopathological features of patients and to evaluate the therapeutic effectiveness of adenovirus-mediated CD40 ligand on ovarian cancer in vitro.
The expression of CD40 on paraffin-embedded ovarian cancer tissues (n=58) and normal ovarian tissues (n=15) was tested by immunohistochemistry, and CD40 expression on ovarian cancer cells derived from fresh surgical specimens was tested by flow cytometry analysis. The apoptosis-inducing effects of adenovirus-mediated CD40 ligand therapy on ovarian cancer cells derived from fresh surgical specimens were analyzed by flow cytometry analysis and TUNEL assay.
CD40 expression was detected in 60.3% (35/58) of paraffin-embedded ovarian cancer tissues and 73.3% (11/15) of fresh ovarian cancer tissues, but not in normal ovarian tissues (n=15). CD40 expression was significantly correlated with FIGO stage of ovarian cancer. Adenovirus-mediated CD40 ligand therapy induced significant apoptosis effects on ovarian cancer cells derived from fresh surgical specimens in vitro compared to null adenovirus vector and phosphate-buffered saline.
Our results suggested the therapeutic potential of adenovirus-mediated CD40 ligand on ovarian cancer, especially on the late stage of ovarian cancer.
检测卵巢癌组织中CD40的表达水平及其与患者临床病理特征的相关性，并评估腺病毒介导的CD40配体对卵巢癌的体外治疗效果。
采用免疫组织化学法检测石蜡包埋的卵巢癌组织（n = 58）和正常卵巢组织（n = 15）中CD40的表达，采用流式细胞术分析新鲜手术标本来源的卵巢癌细胞中CD40的表达。通过流式细胞术分析和TUNEL检测分析腺病毒介导的CD40配体治疗对新鲜手术标本来源的卵巢癌细胞的凋亡诱导作用。
在石蜡包埋的卵巢癌组织中，60.3%（35/58）检测到CD40表达；在新鲜卵巢癌组织中，73.3%（11/15）检测到CD40表达，但在正常卵巢组织（n = 15）中未检测到。CD40表达与卵巢癌的国际妇产科联盟（FIGO）分期显著相关。与空腺病毒载体和磷酸盐缓冲盐水相比，腺病毒介导的CD40配体治疗在体外对新鲜手术标本来源的卵巢癌细胞具有显著的凋亡诱导作用。
我们的结果表明腺病毒介导的CD40配体对卵巢癌具有治疗潜力，尤其是对晚期卵巢癌。
