Motojima K, Kohara N, Furui J, Terada M, Tsunoda T, Nagata Y, Urano K
Second Department of Surgery and Oncology, Nagasaki University School of Medicine, Japan.
Nihon Geka Gakkai Zasshi. 1991 Apr;92(4):453-8.
Regarding to the pancreatic cancer, outcomes of the patients surgically treated have been poor. By using polymerase chain reaction (PCR), paraffin-embedded specimens of the pancreatic carcinoma were confined point mutation in Kirsten (K)-ras codon 12. Then, incidence and type of point mutation of this oncogene and correlative studies with stage, T or N factor of pancreatic cancer were analysed. Extremely high incidence of K-ras gene mutation was shown in present report. The highest mode of point mutation of K-ras oncogene was GGT to GAT coded aspartic acid. Cases without point mutation in K-ras codon 12 were significantly frequent in papillary adenocarcinoma than in tubular type. There were not correlative result among mutation types, stage and T factor of pancreatic cancer. Most patients with pancreatic cancer who survived more than 2 years have not shown mutation to aspartic acid. Four cases including two cases of mucin producing pancreatic cancer did not have point mutation in K-ras codon 12. Pathogenesis of mucin producing cancer can be distinguished from typical pancreatic cancer by detection of point mutation in K-ras codon 12 using PCR.
关于胰腺癌，接受手术治疗的患者预后较差。通过聚合酶链反应（PCR），在胰腺癌石蜡包埋标本中检测到Kirsten（K）-ras密码子12的点突变。然后，分析了该癌基因点突变的发生率和类型，以及与胰腺癌分期、T或N因子的相关性研究。本报告显示K-ras基因突变的发生率极高。K-ras癌基因的最高突变模式是GGT突变为编码天冬氨酸的GAT。K-ras密码子12无点突变的病例在乳头状腺癌中比在管状腺癌中更为常见。胰腺癌的突变类型、分期和T因子之间没有相关性。大多数存活超过2年的胰腺癌患者未出现天冬氨酸突变。包括2例黏液产生性胰腺癌在内的4例患者K-ras密码子12无点突变。通过使用PCR检测K-ras密码子12的点突变，黏液产生性癌的发病机制可与典型胰腺癌相区分。
