[大鼠肺发育过程中 aquaporin 表达的变化]

[Changes of aquaporin expression during lung development in rats].

作者信息

Chen Guo-Bing, Xu Feng, Lu Zhong-Yi, Kuang Feng-Wu

机构信息

Department of Pediatrics, First Hospital of Xiamen Affiliated to Fujian Medical University, Xiamen, Fujian 361003, China.

出版信息

Zhongguo Dang Dai Er Ke Za Zhi. 2008 Aug;10(4):523-6.

PMID:18706178

Abstract

OBJECTIVE

Many studies have shown that tissue development is closely correlated with fluid transport. Aquaporins (AQPs) are a group of cell membrane proteins that actively and selectively transport water. This study aimed to investigate the changes of AQPs expression during lung development in rats in order to elucidate the role of AQPs in the rat lung development.

METHODS

AQP1, AQP3, AQP4 and AQP5 proteins and mRNA in the lung cell membrane were measured by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) respectively in the 20-day-old embryo (E20), 7-day-old newborn rat, and one-month-old young and adult rats. The correlation between AQPs expression and lung development was studied.

RESULTS

With increasing age, the lung development showed a dynamic and successive course, with the most rapid from the fetus to the newborn rat, and then a slowed down afterwards. AQPs mRNA was weakly expressed in the lung of the E20 group. Lung AQPs mRNA and protein increased rapidly after birth until adulthood. The AQPs distribution patterns in the lung were unique with no duplication. There was a positive correlation between AQPs expression and lung development (P<0.05).

CONCLUSIONS

In addition to being involved in the transepithelial transport of water in the lung, AQPs is also related to its development.

摘要

目的

许多研究表明组织发育与液体运输密切相关。水通道蛋白（AQPs）是一类主动且选择性运输水的细胞膜蛋白。本研究旨在探讨大鼠肺发育过程中水通道蛋白表达的变化，以阐明水通道蛋白在大鼠肺发育中的作用。

方法

分别采用免疫组织化学和逆转录 - 聚合酶链反应（RT-PCR）检测20日龄胚胎（E20）、7日龄新生大鼠、1月龄幼鼠及成年大鼠肺细胞膜上的水通道蛋白1（AQP1）、水通道蛋白3（AQP3）、水通道蛋白4（AQP4）和水通道蛋白5（AQP5）的蛋白及mRNA水平。研究水通道蛋白表达与肺发育之间的相关性。