Department of General Surgery, First Affiliated Hospital, Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, PR China.
Biomed Pharmacother. 2010 May;64(5):313-8. doi: 10.1016/j.biopha.2009.12.003. Epub 2010 Jan 8.
New evidence for involvement of aquaporins (AQPs) in cell migration and proliferation adds AQPs to an expanding list of effectors in tumor biology. But there is few report concerning the expression and role of AQPs in human gastric carcinogenesis so far. The aim of this current study was to investigate the expression profile of AQPs in human gastric carcinoma and its significance.
We screened the expression profile of AQP0 approximately AQP12 in gastric adenocarcinoma tissues and corresponding normal mucosa from 89 patients with gastric cancer by reverse transcriptase polymerase chain reaction (RT-PCR), Western blot analysis and immunochemical assay. The relationship between AQPs expression and clinicopathologic characteristics of patients was evaluated.
Based on RT-PCR of 13 AQPs examined, AQP1, 3, 4, 5 and 11 were expressed in human gastric cancers or normal gastric tissues, and AQP3, 4 and 5 exhibited differential expression between human gastric carcinomas and corresponding normal tissues, which was confirmed by Western blot analyses. Immunohistochemical assay showed that AQP4 protein was expressed mainly in the membrane of parietal cell and chief cell in the normal gastric mucosa, and absent in carcinoma tissues. AQP3 and AQP5 were detected remarkably stronger in the carcinoma tissues than that in normal mucosa by immunofluorescence. AQP3 expression in cases with undifferentiated tumor was more than that in cases with well-differentiated tumor. Both AQP3 and AQP5 expression were associated with lymph node metastasis and lymphovascular invasion in patients.
These findings of differential expressions of AQPs and their correlation with clinicopathologic characteristics implicated AQPs might play a role in human gastric carcinogenesis.
水通道蛋白（AQP）在细胞迁移和增殖中的新作用证据将 AQP 添加到肿瘤生物学中不断扩大的效应物列表中。但到目前为止，关于 AQP 在人类胃癌发生中的表达和作用的报道很少。本研究旨在探讨 AQP 在人类胃癌中的表达谱及其意义。
我们通过逆转录聚合酶链反应（RT-PCR）、Western blot 分析和免疫化学检测，从 89 例胃癌患者的胃腺癌组织及其相应的正常黏膜中筛选 AQP0 至 AQP12 的表达谱。评估 AQP 表达与患者临床病理特征的关系。
基于对 13 种 AQP 的 RT-PCR 检测，AQP1、3、4、5 和 11 在人类胃癌或正常胃组织中表达，AQP3、4 和 5 在人类胃癌和相应的正常组织之间表现出差异表达，Western blot 分析证实了这一点。免疫组织化学检测显示，AQP4 蛋白主要在正常胃黏膜壁细胞和主细胞的膜上表达，而在癌组织中不存在。免疫荧光检测显示，AQP3 和 AQP5 在癌组织中的表达明显强于正常黏膜。未分化肿瘤病例的 AQP3 表达高于分化良好的肿瘤病例。AQP3 和 AQP5 的表达与患者的淋巴结转移和血管淋巴管浸润相关。
这些 AQP 的差异表达及其与临床病理特征的相关性表明，AQP 可能在人类胃癌发生中发挥作用。
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