Kuligowski J, Quintás G, Garrigues S, de la Guardia M
Analytical Chemistry Department, Universitat de València, Edifici Jeroni Muñoz, 50th Dr. Moliner, 46100 Burjassot, Spain.
Anal Chim Acta. 2008 Aug 29;624(2):278-85. doi: 10.1016/j.aca.2008.06.055. Epub 2008 Jul 12.
In this work, it has been extended to methanol:water mobile phases, the use of a background correction method for on-line LC-FTIR measurements named Univariate background correction based on the use of a reference spectra matrix (UBC-RSM) and absorbance ratios. It permits to overcome the problem related to spectral changes occurring during the gradient elution, which in the past limited the on-line coupling of LC and FTIR to isocratic elutions. The combined use of the aforementioned background correction technique in on-line isocratic and gradient LC-FTIR, and partial least squares (PLS) has been applied for the search of the critical conditions for polymers. Polyethylenglycol (PEG) has been used as a model example and results found fitted well with previously published ones.
在这项工作中，它已扩展到甲醇：水流动相，使用了一种用于在线液相色谱 - 傅里叶变换红外光谱（LC - FTIR）测量的背景校正方法，称为基于参考光谱矩阵（UBC - RSM）和吸光度比的单变量背景校正。它能够克服与梯度洗脱过程中发生的光谱变化相关的问题，过去这一问题限制了液相色谱和傅里叶变换红外光谱的在线联用只能用于等度洗脱。上述背景校正技术在在线等度和梯度LC - FTIR中的联合使用，以及偏最小二乘法（PLS）已被应用于寻找聚合物的关键条件。聚乙二醇（PEG）已被用作模型示例，发现结果与先前发表的结果非常吻合。
