Carvalho Valdemir Melechco, Nakamura Odete Hirata, Vieira José Gilberto Henriques
Fleury Medicine and Health, Av. Gal. Valdomiro de Lima, 508 São Paulo, SP, CEP 04344-070, Brazil.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Sep 1;872(1-2):154-61. doi: 10.1016/j.jchromb.2008.07.035. Epub 2008 Aug 3.
Quantitation of endogenous steroids is important in the diagnosis of several endocrine disorders. In this study we present a new method for simultaneous quantitation of cortisol, cortisone, 11-desoxycortisol, 21-desoxycortisol, corticosterone, 17-hydroxyprogesterone and 11-desoxycorticosterone in human serum by on-line extraction and LC-MS/MS. Analytes extraction was performed on-line using a 2-position and 6-port valve equipped with a monolithic silica cartridge. After chromatographic separation of all analytes, detection was performed in the multiple reaction monitoring mode using positive atmospheric pressure chemical ionization mode. Total imprecision of the assay ranged from 5.5 to 15.5%. Comparison with immunoassays yielded coefficients of 0.893 for cortisol, 0.848 for 11-desoxycortisol and 0.924 for 17-hydroxyprogesterone. The sensitivity of this method provides meaningful data for patients within normal and elevated range and it may be useful for the diagnosis of a variety of adrenal dysfunctions.
内源性类固醇的定量分析在多种内分泌疾病的诊断中具有重要意义。在本研究中，我们提出了一种通过在线萃取和液相色谱-串联质谱法同时定量人血清中皮质醇、可的松、11-脱氧皮质醇、21-脱氧皮质醇、皮质酮、17-羟孕酮和11-脱氧皮质酮的新方法。使用配备整体硅胶柱的二位六通阀在线进行分析物萃取。所有分析物经色谱分离后，采用正大气压化学电离模式在多反应监测模式下进行检测。该测定方法的总不精密度范围为5.5%至15.5%。与免疫测定法比较，皮质醇的系数为0.893，11-脱氧皮质醇为0.848，17-羟孕酮为0.924。该方法的灵敏度可为正常范围和升高范围内的患者提供有意义的数据，可能有助于诊断多种肾上腺功能障碍。
