Helmy Mohamed, Baddar Doa, El'Masry Mohamed Hisham
Department of Biotechnology, Institute of Graduate Studies and Research (IGSR), Alexandria University, Alexandria, 21526, Egypt.
Biochemistry (Mosc). 2008 Jul;73(7):776-82. doi: 10.1134/s0006297908070055.
Bacterial colonies were isolated from different Egyptian soil samples. From these isolates, one bacterial species was found to produce siderophore. Using classical and biochemical identification methods, the siderophore producing isolate was identified as Pseudomonas fluorescens. Based on the affinity of siderophores for metal ions, an affinity chromatography system was designed for the purification of the siderophore in one step. It was possible to isolate 25 mg siderophore per liter of culture media. The purified siderophore was found to exist in two forms of approximately 30 and 90 kD. They are believed to be polymers of several siderophore molecules. Both forms were found to be active against the pathogen Erwinia carotovora var. carotovora, the causal bacteria of soft rot disease on potato tubers. The advantage of this method over other purification methods is that it uses metal ion so it can be applied for the purification of the known types of siderophores. Moreover, the purification is based on affinity chromatography, so the siderophore purity state permits several biotechnological applications without further treatments.
从不同的埃及土壤样本中分离出细菌菌落。在这些分离菌株中，发现有一种细菌能产生铁载体。通过经典和生化鉴定方法，将产生铁载体的分离菌株鉴定为荧光假单胞菌。基于铁载体对金属离子的亲和力，设计了一种亲和色谱系统用于一步纯化铁载体。每升培养基能够分离出25毫克铁载体。发现纯化后的铁载体存在两种形式，分子量分别约为30 kDa和90 kDa。它们被认为是几种铁载体分子的聚合物。两种形式的铁载体均被发现对胡萝卜软腐欧文氏菌胡萝卜软腐变种具有活性，该菌是马铃薯块茎软腐病的致病菌。这种方法相对于其他纯化方法的优势在于它使用金属离子，因此可用于已知类型铁载体的纯化。此外，纯化基于亲和色谱，所以铁载体的纯度状态允许进行多种生物技术应用而无需进一步处理。
