Cheng Andrew S, Yau Terrence M
Division of Cardiovascular Surgery, Toronto General Hospital, Toronto, Ontario, Canada.
Semin Thorac Cardiovasc Surg. 2008 Summer;20(2):94-101. doi: 10.1053/j.semtcvs.2008.04.003.
Within the last few years, it has become evident that the beneficial effect of cell transplantation on ventricular function and myocardial perfusion is in large part mediated through paracrine effects on the host myocardium. Studies in which medium conditioned by cultured cells, usually mesenchymal stem cells, were injected into infarcted animal hearts have provided definitive evidence of this mechanism of action. Paracrine effects of the donor cells include but are not limited to angiogenesis, mobilization of both circulating and bone-marrow-derived stem cells, activation of cardiac-resident stem cells (CRSCs), and stabilization of the extracellular matrix (ECM). These paracrine effects can be augmented by transplantation of cells modified to express therapeutically useful transgenes, or by preconditioning through hypoxic or pharmacologic means. Strategies to enhance the paracrine effects of cell transplantation may thus be employed in the next generation of cell therapies, with greater functional benefit.
在过去几年中，已经很明显，细胞移植对心室功能和心肌灌注的有益作用在很大程度上是通过对宿主心肌的旁分泌作用介导的。将培养细胞（通常是间充质干细胞）条件培养基注入梗死动物心脏的研究为这种作用机制提供了确凿证据。供体细胞的旁分泌作用包括但不限于血管生成、循环和骨髓来源干细胞的动员、心脏驻留干细胞（CRSCs）的激活以及细胞外基质（ECM）的稳定。通过移植经修饰以表达治疗有用转基因的细胞，或通过缺氧或药物预处理，可以增强这些旁分泌作用。因此，增强细胞移植旁分泌作用的策略可能会应用于下一代细胞治疗中，带来更大的功能益处。
