Guo Weiwei, Yuan Jipei, Li Bingling, Du Yan, Ying Erbo, Wang Erkang
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Analyst. 2008 Sep;133(9):1209-13. doi: 10.1039/b806301j. Epub 2008 Jul 28.
A unique multilabeling at a single-site protocol of the Ru(bpy)(3)(2+) electrochemiluminescence (ECL) system is proposed. Nanoparticles (NPs) were used as assembly substrates to enrich ECL co-reactants of Ru(bpy)(3)(2+) to construct nanoscale-enhanced ECL labels. Two different kinds of NP substrates [including semiconductor NPs (CdTe) and noble metal NPs (gold)] capped with 2-(dimethylamino)ethanethiol (DMAET) [a tertiary amine derivative which is believed to be one of the most efficient of co-reactants of the Ru(bpy)(3)(2+) system] were synthesized through a simple one-pot synthesis method in aqueous media. Although both CdTe and gold NPs realized the enrichment of ECL co-reactants, they presented entirely different ECL performances as nanoscale ECL co-reactants of Ru(bpy)(3)(2+). The different effects of these two NPs on the ECL of Ru(bpy)(3)(2+) were studied. DMAET-capped CdTe NPs showed enormous signal amplification of Ru(bpy)(3)(2+) ECL, whereas DMAET-capped gold NPs showed a slight quenching effect of the ECL signal. DMAET-capped CdTe NPs can be considered to be excellent nanoscale ECL labels of the Ru(bpy)(3)(2+) system, as even a NP solution sample of 10(-18) M was still detectable after an electrostatic self-assembly concentration process. DMAET-capped CdTe NPs were further applied in the construction of aptamer-based biosensing system for proteins and encouraging results were obtained.
提出了一种基于钌（联吡啶）（3）（2+）电化学发光（ECL）体系的单位点独特多标记方案。纳米颗粒（NPs）用作组装底物，以富集钌（联吡啶）（3）（2+）的ECL共反应剂，构建纳米级增强ECL标记。通过简单的一锅法在水介质中合成了两种不同的NP底物[包括半导体NP（CdTe）和贵金属NP（金）]，它们均用2-（二甲基氨基）乙硫醇（DMAET）[一种叔胺衍生物，被认为是钌（联吡啶）（3）（2+）体系中最有效的共反应剂之一]进行了封端。尽管CdTe和金NP都实现了ECL共反应剂的富集，但作为钌（联吡啶）（3）（2+）的纳米级ECL共反应剂，它们呈现出完全不同的ECL性能。研究了这两种NP对钌（联吡啶）（3）（2+）ECL的不同影响。DMAET封端的CdTe NP显示出钌（联吡啶）（3）（2+）ECL的巨大信号放大，而DMAET封端的金NP显示出ECL信号的轻微猝灭作用。DMAET封端的CdTe NP可被认为是钌（联吡啶）（3）（2+）体系出色的纳米级ECL标记，因为即使经过静电自组装浓缩过程后，10（-18）M的NP溶液样品仍可检测到。DMAET封端的CdTe NP进一步应用于构建基于适体的蛋白质生物传感系统，并获得了令人鼓舞的结果。
