Xuming Wang, Fengqing Gao, Linglan Ma, Jie Liu, Sitao Yin, Ping Yang, Fashui Hong
Medical College of Suzhou University, Suzhou, 215123, People's Republic of China.
Biol Trace Elem Res. 2008 Winter;126(1-3):280-9. doi: 10.1007/s12011-008-8203-y. Epub 2008 Aug 17.
Being a proven photocatalyst, nano-anatase is capable of undergoing electron transfer reactions under light. In previous studies, we had proven that nano-anatase improved photosynthesis and greatly promoted spinach growth. However, the molecular mechanisms of carbon reaction promoted by nano-anatase are still not clearly understood. In the present paper, reverse transcription polymerase chain reaction and Northern blotting were carried out, and the results showed that the expression of ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit (rbcS) and Rubisco large subunit (rbcL) messenger RNA (mRNA) in the nano-anatase-treated spinach were obviously promoted, respectively, while the effects of bulk TiO2 were not so significant. Accordingly, the protein expression of Rubisco from the nano-anatase-treated spinach was increased by 40% compared with control. Further analysis indicated that the activity of Rubisco in the nano-anatase-treated spinach was significantly higher than the control, by up to 2.33 times, and bulk TiO2 treatment had no such significant effects. Together, one of the molecular mechanisms of carbon reaction promoted by nano-anatase is that the nano-anatase treatment results in the enhancement of Rubisco mRNA amounts, the protein levels, and activity of Rubisco, thereby leading to the improvement of Rubisco carboxylation and the high rate of photosynthetic carbon reaction.
作为一种经过验证的光催化剂，纳米锐钛矿能够在光照下进行电子转移反应。在先前的研究中，我们已经证明纳米锐钛矿能改善光合作用并极大地促进菠菜生长。然而，纳米锐钛矿促进碳反应的分子机制仍不清楚。在本文中，进行了逆转录聚合酶链反应和Northern印迹分析，结果表明，纳米锐钛矿处理的菠菜中，1,5-二磷酸核酮糖羧化酶/加氧酶（Rubisco）小亚基（rbcS）和Rubisco大亚基（rbcL）信使核糖核酸（mRNA）的表达分别明显受到促进，而块状二氧化钛的作用则不那么显著。因此，纳米锐钛矿处理的菠菜中Rubisco的蛋白质表达与对照相比增加了40%。进一步分析表明，纳米锐钛矿处理的菠菜中Rubisco的活性明显高于对照，高达2.33倍，而块状二氧化钛处理则没有如此显著的效果。总之，纳米锐钛矿促进碳反应的分子机制之一是，纳米锐钛矿处理导致Rubisco mRNA量、蛋白质水平和Rubisco活性增强，从而导致Rubisco羧化作用改善和光合碳反应速率提高。
