Kreuter Alexander, Bischoff Sebastian, Skrygan Marina, Wieland Ulrike, Brockmeyer Norbert H, Stücker Markus, Altmeyer Peter, Gambichler Thilo
Department of Dermatology, Ruhr University Bochum, Gudrunstrasse 56, D-44791 Bochum, Germany.
Arch Dermatol. 2008 Aug;144(8):1011-6. doi: 10.1001/archderm.144.8.1011.
To investigate the presence of human herpesvirus 8 (HHV-8) in lesional skin of German patients with large-plaque parapsoriasis (LPP) or mycosis fungoides (MF). The pathogenetic relevance of HHV-8 in cutaneous T-cell lymphoma is controversial. Recently, a highly significant association of HHV-8 in LPP was found, which suggests a role in the pathogenesis of this disease.
Retrospective study of the presence of HHV-8 in German patients with lymphoproliferative diseases.
Dermatologic clinic at a university hospital of the Ruhr University Bochum, Bochum, Germany.
Fifty-three patients treated for lymphoproliferative skin diseases were included in the study, including 14 patients with LPP, 31 with different stages of MF, and 8 with lymphomatoid papulosis (LyP). Twenty-three patients with Kaposi sarcoma (KS) made up the positive control group, and 10 patients with atopic dermatitis served as negative controls.
The presence of HHV-8 was analyzed from paraffin-embedded lesional tissue samples using a nested polymerase chain reaction for the open reading frame (ORF) 26 and with immunohistochemical staining for the latency-associated nuclear antigen (LANA) encoded by ORF 73.
A high association of HHV-8 infection in both lymphoproliferative skin diseases was observed: 87% of LPP and 70% of MF tissue samples tested positive for HHV-8 DNA from ORF 26. However, HHV-8 was not detectable in LPP and MF by using the immunohistochemical marker LANA.
A virus unambiguously associated with KS, HHV-8 was frequently detected at low amounts in LPP and MF specimens. However, based on the methods of HHV-8 detection used in this study, no conclusion can be drawn on the etiologic and pathogenetic role of HHV-8 in these diseases.
调查德国大斑块状副银屑病（LPP）或蕈样肉芽肿（MF）患者皮损中人类疱疹病毒8型（HHV-8）的存在情况。HHV-8在皮肤T细胞淋巴瘤发病机制中的相关性存在争议。最近，发现HHV-8与LPP存在高度显著关联，这表明其在该疾病发病机制中发挥作用。
对德国淋巴增殖性疾病患者中HHV-8的存在情况进行回顾性研究。
德国波鸿鲁尔大学附属医院皮肤科诊所。
纳入53例接受淋巴增殖性皮肤病治疗的患者，包括14例LPP患者、31例不同分期的MF患者和8例淋巴瘤样丘疹病（LyP）患者。23例卡波西肉瘤（KS）患者组成阳性对照组，10例特应性皮炎患者作为阴性对照组。
使用针对开放阅读框（ORF）26的巢式聚合酶链反应，以及针对由ORF 73编码的潜伏相关核抗原（LANA）的免疫组织化学染色，从石蜡包埋的皮损组织样本中分析HHV-8的存在情况。
在两种淋巴增殖性皮肤病中均观察到HHV-8感染的高度关联：87%的LPP和70%的MF组织样本经ORF 26检测HHV-8 DNA呈阳性。然而，通过免疫组织化学标志物LANA在LPP和MF中未检测到HHV-8。
HHV-8是一种与KS明确相关的病毒，在LPP和MF标本中经常检测到少量病毒。然而，基于本研究中使用的HHV-8检测方法，无法就HHV-8在这些疾病中的病因和发病机制作用得出结论。
