Katano H, Suda T, Morishita Y, Yamamoto K, Hoshino Y, Nakamura K, Tachikawa N, Sata T, Hamaguchi H, Iwamoto A, Mori S
Department of Pathology, Institute of Medical Science, University of Tokyo, Japan.
Mod Pathol. 2000 Jan;13(1):77-85. doi: 10.1038/modpathol.3880012.
Human herpesvirus type 8 (HHV-8; Kaposi's sarcoma-associated herpesvirus) is a recently isolated human herpesvirus frequently identified in Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. Here we report three cases of HHV-8-bearing solid lymphomas that occurred in AIDS patients (Cases 1-3). All three patients were homosexual men presenting extranodal masses in the lungs (Case 1) or skin (Cases 2 and 3), together with the presence of Kaposi's sarcoma (Case 1), primary effusion lymphoma (Case 2), or multicentric Castleman's disease (Case 3). These solid lymphomas exhibited anaplastic large cell morphology and expressed CD30, corresponding to the recent diagnostic criteria of anaplastic large cell lymphoma (ALCL). The chromosomal translocation t(2;5)-associated chimeric protein p80NPM/ALK was not observed in any of these cases. HHV-8 was detected in all of these cases by polymerase chain reaction, immunohistochemistry of HHV-8-encoded ORF73 protein, and in situ hybridization of T1.1. Epstein-Barr virus was detected only in Cases 2 and 3 by in situ hybridization. It is interesting that inoculation of a cell line obtained from a primary effusion lymphoma cell in Case 2 to severe combined immunodeficiency mice produced HHV-8-positive and Epstein-Barr virus-negative tumors in inoculated sites. These tumor cells exhibited phenotypes of ALCL that were identical to the subcutaneous tumor cells of this particular patient. These findings clearly show that HHV-8 can associate with solid lymphomas and that it can take anaplastic large cell morphology. Those lymphomas should be distinguished from the classical ALCL as were defined by the revised European-American classification of lymphoid neoplasms even though morphology and a part of immunophenotype mimic that of classical ALCL.
人疱疹病毒8型（HHV-8；卡波西肉瘤相关疱疹病毒）是一种最近分离出的人疱疹病毒，在卡波西肉瘤、原发性渗出性淋巴瘤和多中心性Castleman病中经常被发现。在此，我们报告3例发生于艾滋病患者的携带HHV-8的实体淋巴瘤病例（病例1-3）。所有3例患者均为男同性恋者，分别出现肺部（病例1）或皮肤（病例2和3）的结外肿块，同时伴有卡波西肉瘤（病例1）、原发性渗出性淋巴瘤（病例2）或多中心性Castleman病（病例3）。这些实体淋巴瘤表现为间变性大细胞形态，表达CD30，符合间变性大细胞淋巴瘤（ALCL）的最新诊断标准。在这些病例中均未观察到与染色体易位t(2;5)相关的嵌合蛋白p80NPM/ALK。通过聚合酶链反应、HHV-8编码的ORF73蛋白免疫组化以及T1.1原位杂交，在所有这些病例中均检测到HHV-8。仅通过原位杂交在病例2和3中检测到EB病毒。有趣的是，将病例2中原发性渗出性淋巴瘤细胞系接种到严重联合免疫缺陷小鼠后，在接种部位产生了HHV-8阳性且EB病毒阴性的肿瘤。这些肿瘤细胞表现出与该特定患者皮下肿瘤细胞相同的ALCL表型。这些发现清楚地表明，HHV-8可与实体淋巴瘤相关联，并且可呈现间变性大细胞形态。尽管这些淋巴瘤的形态和部分免疫表型与经典ALCL相似，但仍应将其与欧美淋巴样肿瘤修订分类所定义的经典ALCL区分开来。
