罗塞塔石碑法。

The Rosetta stone method.

作者信息

Date Shailesh V

机构信息

PENN Center for Bioinformatics, Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.

出版信息

Methods Mol Biol. 2008;453:169-80. doi: 10.1007/978-1-60327-429-6_7.

DOI:10.1007/978-1-60327-429-6_7

PMID:18712302

Abstract

Analysis of amino acid sequences from different organisms often reveals cases in which two or more proteins encoded for separately in a genome also appear as fusions, either in the same genome or that of some other organism. Such fusion proteins, termed Rosetta stone sequences, help link disparate proteins together, and suggest the likelihood of functional interactions between the linked entities, describing local and global relationships within the proteome. These relationships help us understand the role of proteins within the context of their associations, and facilitate assignment of putative functions to uncharacterized proteins based on their linkages with proteins of known function.

摘要

对来自不同生物体的氨基酸序列进行分析，常常会发现这样的情况：在一个基因组中分别编码的两种或更多种蛋白质，在同一基因组或其他生物体的基因组中也以融合形式出现。这种融合蛋白，被称为罗塞塔石碑序列，有助于将不同的蛋白质联系在一起，并暗示了所连接实体之间功能相互作用的可能性，描述了蛋白质组内的局部和全局关系。这些关系有助于我们理解蛋白质在其关联背景下的作用，并基于未表征蛋白质与已知功能蛋白质的联系，促进对其假定功能的分配。