Alhebshi Hasen M, Pant Ishita, Kaur Gurjeet, Hashim Hasnah, Mabruk Mohamed J E M F
Institute of Medicine, University of Brunei Darussalam, Jalan Tungku Link, Gadong BE1410, Brunei Darussalam.
Asian Pac J Cancer Prev. 2008 Apr-Jun;9(2):291-4.
The methylthioadenosine phosphorylase (MTAP) gene is a tumour suppressor gene, located on chromosome 9p21, 100 kb telomeric of the p15 and p16 genes, which are often deleted in tumor cells. The role of MTAP protein expression in the genesis of cutaneous squamous cell carcinoma (SCC) is currently not known. In a previous study we have shown the frequent occurrence of allelic imbalance/loss of heterozygosity (AI/LOH) in cutaneous SCCs using AI/LOH markers flanking the p15, p16, and MTAP genes and demonstrated reduction in p15 and p16 protein expression in comparison to normal human skin. The present study is a continuation to our previous studies, aimed at determining possible roles played by MTAP protein expression in the genesis of cutaneous SCC. The expression of MTAP protein was detected using immunohistochemical approach in 109 micro array cutaneous SCC and 20 normal human skin tissue samples. The expression of MTAP was not significantly different in the cutaneous SCC cases as compared with normal human skin. This may indicate that MTAP protein expression does not contribute to the genesis of cutaneous SCC.
甲硫腺苷磷酸化酶（MTAP）基因是一种肿瘤抑制基因，位于9号染色体p21区，在p15和p16基因的端粒方向100 kb处，这两个基因在肿瘤细胞中常发生缺失。MTAP蛋白表达在皮肤鳞状细胞癌（SCC）发生中的作用目前尚不清楚。在之前的一项研究中，我们使用位于p15、p16和MTAP基因两侧的等位基因失衡/杂合性缺失（AI/LOH）标记，显示皮肤SCC中频繁出现AI/LOH，并证明与正常人类皮肤相比，p15和p16蛋白表达降低。本研究是我们之前研究的延续，旨在确定MTAP蛋白表达在皮肤SCC发生中可能发挥的作用。采用免疫组化方法检测了109例微阵列皮肤SCC和20例正常人类皮肤组织样本中MTAP蛋白的表达。与正常人类皮肤相比，皮肤SCC病例中MTAP的表达无显著差异。这可能表明MTAP蛋白表达对皮肤SCC的发生没有作用。
