系统性红斑狼疮患者中DNA反应性B细胞的鉴定
Identification of DNA-reactive B cells in patients with systemic lupus erythematosus.
作者信息
Zhang Jie, Jacobi Annett M, Mackay Meggan, Aranow Cynthia, Wang Tao, Chinnasamy Prameladevi, Diamond Betty
机构信息
Feinstein Institute for Medical Research, North Shore-Long Island Jewish Health System, Manhasset, New York 11030, USA.
出版信息
J Immunol Methods. 2008 Sep 30;338(1-2):79-84. doi: 10.1016/j.jim.2008.07.015. Epub 2008 Aug 17.
Abstract
Autoreactive B cells play a central role in systemic lupus erythematosus (SLE). Characterization of DNA-reactive B cells in the blood of lupus patients has been limited by the low frequency of the population. Using a tetrameric configuration of a peptide mimetope of DNA, we identified peptide-reactive B cells in peripheral blood. Antibodies derived from these B cells bound to peptide and were largely cross-reactive to dsDNA. This methodology enables us to track the development of autoreactive B cells, which recognize peptide and dsDNA, in individual patients with SLE and permits the isolation of autoreactive B cells for further characterization.
摘要
自身反应性B细胞在系统性红斑狼疮（SLE）中起核心作用。狼疮患者血液中DNA反应性B细胞的特征一直受到该细胞群体低频率的限制。利用DNA肽模拟表位的四聚体结构，我们在外周血中鉴定出了肽反应性B细胞。源自这些B细胞的抗体与肽结合，并且在很大程度上与双链DNA交叉反应。这种方法使我们能够追踪SLE个体患者中识别肽和双链DNA的自身反应性B细胞的发育情况，并允许分离自身反应性B细胞以进行进一步表征。
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