Feng Ying-Qian, Zuo Xue-Lan, Li Rui-Fang, Zhang Ke-Jian, Chen Fei, Xiao Hui
Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Aug;16(4):790-3.
The objective of this study was to investigate the protection by naringenin against doxorubicin-induced oxidative damage in normal blood cells. Inhibiting effects of naringenin, doxorubicin and naringenin combined with doxorubicind on K562 cells and polymorphonuclear leukocytes were detected with MTT method, the level of reactive oxygen species (ROS) and lipid peroxidation (MDA), the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were examined with spectrophotometric method in the K562 cells and polymorphonuclear leukocytes. The results indicated that the proliferation of K562 cells was not inhibited by the cytotoxicity of doxorubicin in combination of naringenin with doxorubicin. As compared with the doxorubicin, the addition of naringenin after doxorubicin for 1 hour, the levels of reactive oxygen species (ROS) and lipid peroxidation (MDA) obviously decreased, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) obviously increased in the polymorphonuclear leukocytes, but these were not changed obviously in K562 cells. It is concluded naringenin can protect against doxorubicin-induced oxidative damage in normal blood cells. The mechanism of naringenin may be elevating activities of antioxidant enzyme and degrading oxidative production level in normal blood cells, and meanswhile decreasing level of oxidative products.
本研究的目的是探讨柚皮素对阿霉素诱导的正常血细胞氧化损伤的保护作用。采用MTT法检测柚皮素、阿霉素及柚皮素与阿霉素联合作用对K562细胞和多形核白细胞的抑制作用，用分光光度法检测K562细胞和多形核白细胞中活性氧（ROS）水平、脂质过氧化产物丙二醛（MDA）含量、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性。结果表明，柚皮素与阿霉素联合作用时，阿霉素的细胞毒性未抑制K562细胞的增殖。与阿霉素组相比，阿霉素作用1小时后加入柚皮素，多形核白细胞中活性氧（ROS）水平、脂质过氧化产物丙二醛（MDA）含量明显降低，超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性明显升高，而K562细胞中上述指标变化不明显。结论：柚皮素可保护正常血细胞免受阿霉素诱导的氧化损伤。其机制可能是提高正常血细胞中抗氧化酶的活性，降低氧化产物水平，同时降解氧化产物。
