Gagoś Mariusz, Gruszecki Wiesław I
Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University, 20-031 Lublin, Poland.
Biophys Chem. 2008 Oct;137(2-3):110-5. doi: 10.1016/j.bpc.2008.08.001. Epub 2008 Aug 7.
Amphotericin B (AmB) is a life-saving antibiotic, used to treat deep-seated mycotic infections. Both the therapeutic and toxic side effects of AmB are directly dependent on its molecular organization. Organization of AmB was studied in monocomponent monomolecular layers formed at the argon-water interface, by means of polarized and non-polarized electronic absorption spectroscopy and analyzed in terms of the exciton splitting theory. The results provide direct indication that AmB forms spontaneously dimers that can be assembled into molecular structures characterized by homogeneous orientational distribution in the monolayer, interpreted as cylindrical pores. The structures are not stable at surface pressures higher than 20 mN/m and therefore dimers are concluded as abundant molecular organization forms of AmB in biomembranes. Possibility of stabilization of the cylindrical structures, at higher surface pressures, by other molecules, e.g. sterols, is also discussed.
两性霉素B（AmB）是一种挽救生命的抗生素，用于治疗深部真菌感染。AmB的治疗作用和毒副作用都直接取决于其分子结构。通过偏振和非偏振电子吸收光谱法，在氩气-水界面形成的单组分单分子层中研究了AmB的结构，并根据激子分裂理论进行了分析。结果直接表明，AmB能自发形成二聚体，这些二聚体可组装成分子结构，其特征是在单分子层中具有均匀的取向分布，可解释为圆柱形孔。这些结构在高于20 mN/m的表面压力下不稳定，因此得出结论，二聚体是AmB在生物膜中丰富的分子组织形式。还讨论了通过其他分子（如甾醇）在更高表面压力下稳定圆柱形结构的可能性。
