Zhang Wenwei, Luo Yuping, Gong Xi, Zeng Wenhong, Li Siguang
College of Life Sciences, Nanchang University, Nanchang 330031, PR China.
Comput Biol Chem. 2009 Feb;33(1):84-93. doi: 10.1016/j.compbiolchem.2008.07.006. Epub 2008 Jul 15.
MicroRNAs (miRNAs) are a new family of small RNA molecules known in animals and plants, whose conservation among species suggests that they bear conserved biological functions. So far, little is known about miRNA in Solanum tuberosum species. Using previously known miRNAs from Arabidopsis, rice and other plant species against expressed sequence tags (ESTs), genomic survey sequence (GSS) and nucleotide databases, we identified 48 potential miRNAs in S. tuberosum. These potato miRNAs may regulate 186 potential targets, which are involved in floral, leaf, root, and stem development, signal transduction, metabolism pathways, and stress responses. To validate the prediction of miRNAs in potato, we performed a RT-PCR analysis and found that potato miRNAs have diverse expression patterns during development.
微小RNA（miRNA）是动植物中已知的一类新的小RNA分子，其在物种间的保守性表明它们具有保守的生物学功能。到目前为止，关于马铃薯物种中的miRNA知之甚少。利用先前已知的来自拟南芥、水稻和其他植物物种的miRNA与表达序列标签（EST）、基因组调查序列（GSS）和核苷酸数据库进行比对，我们在马铃薯中鉴定出了48个潜在的miRNA。这些马铃薯miRNA可能调控186个潜在靶标，这些靶标参与花、叶、根和茎的发育、信号转导、代谢途径以及应激反应。为了验证马铃薯中miRNA的预测结果，我们进行了逆转录聚合酶链反应（RT-PCR）分析，发现马铃薯miRNA在发育过程中具有多种表达模式。
