Kapfhammer J P
Brain Research Institute, University of Zürich, Switzerland.
Anal Biochem. 1991 Mar 2;193(2):197-9. doi: 10.1016/0003-2697(91)90008-h.
A method that allows the use of immunoblots as a screening test for large numbers of samples is described. Preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis is run to a separation distance of 2 cm and the samples are blotted onto nitrocellulose. One-millimeter-wide strips are cut and immobilized on double-sided tape. Adjacent strips are separated by plastic rods. After incubation with the first antibody, whole slides are processed together as in histology. The method has been successfully applied to the primary screening of hybridoma supernatants and is generally applicable in situations where large numbers of samples must be tested in a short time.
本文描述了一种可将免疫印迹法用作大量样品筛选试验的方法。进行制备性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，使分离距离达到2厘米，然后将样品转印到硝酸纤维素膜上。将膜切成1毫米宽的条带，用双面胶带固定。相邻条带用塑料棒隔开。与一抗孵育后，将整个载玻片按照组织学方法一起处理。该方法已成功应用于杂交瘤细胞培养上清液的初步筛选，并且通常适用于需要在短时间内检测大量样品的情况。
