Gardezi Abeeha Z, Ziaei Yalda Z, Marashi Sahar M
Second Year Medical Students Dubai Medical College for Girls, Dubai, UAE.
J Crit Care. 2008 Sep;23(3):440-4. doi: 10.1016/j.jcrc.2008.06.007.
Leptin is a hormone mainly produced by adipose tissue. It acts on both energy intake and expenditure to maintain relative stability of body weight and energy storage over long period of time. Variation in size of the tetranucleotide repeat located at the 3' end of leptin gene (LEP3'HVR) can influence leptin expression.
To predict the association of LEP3'HVR and obesity.
A case control study consisting of 40 individuals with normal BMI (control group) and 35 individuals with abnormal BMI. LEP3'HVR polymorphic region was amplified by polymerase chain reaction, and fragments were analyzed by agarose gel electrophoresis. Statistical analysis was done using SPSS (SPSS, Chicago, Ill).
The frequency of LEP3'HVR class I was 52.8% in individuals with abnormal BMI versus 42.5% in the individuals with normal BMI. The homozygous class I (I/I) genotype was identified in 40% of the individuals with abnormal BMI vs 25% of the control group. Individuals with I/I genotypes showed a higher prevalence of obesity when compared with homozygous class II/II genotype (odds ratio, 1.9; 95% confidence interval, 0.6-5.6).
Class (I/I) genotype of LEP3'HVR was associated with increase risk of obesity.
瘦素是一种主要由脂肪组织产生的激素。它作用于能量摄入和消耗，以长期维持体重和能量储存的相对稳定。位于瘦素基因（LEP3'HVR）3'端的四核苷酸重复序列大小的变异可影响瘦素表达。
预测LEP3'HVR与肥胖的关联。
一项病例对照研究，包括40名体重指数（BMI）正常的个体（对照组）和35名BMI异常的个体。通过聚合酶链反应扩增LEP3'HVR多态性区域，并通过琼脂糖凝胶电泳分析片段。使用SPSS（SPSS，伊利诺伊州芝加哥）进行统计分析。
BMI异常个体中LEP3'HVR I类的频率为52.8%，而BMI正常个体中为42.5%。BMI异常个体中有40%鉴定为纯合I类（I/I）基因型，而对照组为25%。与纯合II/II基因型相比，I/I基因型个体的肥胖患病率更高（优势比，1.9；95%置信区间，0.6 - 5.6）。
LEP3'HVR的I类（I/I）基因型与肥胖风险增加相关。
