一种用于检测β2-糖蛋白I的夹心酶联免疫吸附测定法。
A sandwich enzyme-linked immunosorbent assay for beta 2-glycoprotein I.
作者信息
Sansom P A, Marlow C T, Lapsley M, Flynn F V
机构信息
Department of Chemical Pathology, University College and Middlesex School of Medicine, London, UK.
出版信息
Ann Clin Biochem. 1991 May;28 ( Pt 3):283-9. doi: 10.1177/000456329102800315.
A solid-phase sandwich enzyme-linked immunosorbent assay for determining beta 2-glycoprotein I in urine has been developed. It has a working concentration range of 5-40 micrograms/L and a detection limit of approximately 1.4 micrograms/L. The within-plate coefficient of variation (CV) falls between 1.4% and 2.1%, and the between-batch CV ranges from 5.2 to 6.0%. Recovery of beta 2-glycoprotein I added to urine varies between 96 and 110%. The assay can also be used for determining beta 2-glycoprotein I in serum.
已开发出一种用于测定尿液中β2-糖蛋白I的固相夹心酶联免疫吸附测定法。其工作浓度范围为5-40微克/升，检测限约为1.4微克/升。板内变异系数（CV）在1.4%至2.1%之间，批间CV范围为5.2%至6.0%。添加到尿液中的β2-糖蛋白I的回收率在96%至110%之间。该测定法也可用于测定血清中的β2-糖蛋白I。
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