Yang X, Sun Y, Zhou J, Huang P, Huang C, Xu X, Li C, Gotay J, Chen L, Deng C
Institute of Biotechnology, Beijing, China.
Sci China C Life Sci. 2001 Apr;44(2):121-9. doi: 10.1007/BF02879316.
Smad5 is an intracellular transducer of TGF-beta signals. Targeted disruption of murine Smad5 gene resulted in embryonic lethal. To study the function of Smad5 in organgenesis, we generated Smad5 double knockout ES cells by homologous recombination. We deleted the neo gene of the Smad5 targeted ES cells using Cre-LoxP system. Smad5 double knockout ES cells were obtained by transfecting the targeted ES cells using the same targeting construct. The results of chimeric study showed that Smad5 might play an important role during the development of heart and neural tube.Smad5 double knockout ES cells formed teratoma when injected subcutaneously into nude mice. They differentiated into several types of cells, including neural cells, muscle cells, chondrocytes, endothelial cells and glandaceous cells. Smad5 double knockout ES cells are useful for studying the function of Smad5 mediated TGF-beta during the organgenesis and the in vitro differentiation of ES cells.
Smad5是转化生长因子-β（TGF-β）信号的细胞内转导分子。小鼠Smad5基因的靶向破坏导致胚胎致死。为了研究Smad5在器官发生中的功能，我们通过同源重组产生了Smad5双敲除胚胎干细胞（ES细胞）。我们使用Cre-LoxP系统删除了Smad5靶向ES细胞的neo基因。通过使用相同的靶向构建体转染靶向ES细胞获得了Smad5双敲除ES细胞。嵌合体研究结果表明，Smad5可能在心脏和神经管发育过程中发挥重要作用。Smad5双敲除ES细胞皮下注射到裸鼠体内时形成畸胎瘤。它们分化为几种类型的细胞，包括神经细胞、肌肉细胞、软骨细胞、内皮细胞和腺细胞。Smad5双敲除ES细胞可用于研究Smad5介导的TGF-β在器官发生和ES细胞体外分化过程中的功能。
