Won Dong Il, Suh Jang Soo
Department of Clinical Pathology, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
Cytometry B Clin Cytom. 2009 Mar;76(2):135-41. doi: 10.1002/cyto.b.20448.
Hereditary spherocytosis (HS) is the most common cause of inherited chronic hemolysis. The various tests developed for HS screening have many shortcomings. The purpose of this study was to develop a new, simple, reliable test using flow cytometry.
In this new test, deionized water, a hemolysis-inducing agent, is spiked to a red cell suspension during acquisition, and the count of red cells is measured sequentially in real-time using flow cytometry. In this study, the optimal parameters for this test were established, and the discriminatory power of the adopted protocol was verified among several groups with various degrees of osmotic fragility (OF).
The established protocol successfully discriminated between blood analyzed immediately and blood stored for 24 h (P < 0.00001). With healthy individuals as a reference, the OF measured by this protocol was significantly decreased in patients with iron deficiency anemia (P = 0.030) and was significantly increased in HS patients (P = 1.1 x 10(-9)). This new test found all 11 HS patients to have increased OF.
This new test was verified to be simple, quantitative, objective, and cost-effective for the measurement of OF. We suggest this test as another effective approach for HS screening, although its assay performance will require further verification through more clinical trials.
遗传性球形红细胞增多症（HS）是遗传性慢性溶血最常见的病因。为HS筛查开发的各种检测方法存在诸多缺点。本研究的目的是利用流式细胞术开发一种新的、简单、可靠的检测方法。
在这项新检测中，在采集过程中将溶血诱导剂去离子水加入红细胞悬液中，并使用流式细胞术实时顺序测量红细胞计数。在本研究中，确定了该检测的最佳参数，并在几组具有不同程度渗透脆性（OF）的人群中验证了所采用方案的鉴别能力。
既定方案成功区分了立即分析的血液和储存24小时的血液（P < 0.00001）。以健康个体为参照，该方案测得的缺铁性贫血患者的OF显著降低（P = 0.030），而HS患者的OF显著升高（P = 1.1×10⁻⁹）。这项新检测发现所有11例HS患者的OF均升高。
经证实，这项新检测在测量OF方面简单、定量、客观且具有成本效益。我们建议将这项检测作为HS筛查的另一种有效方法，尽管其检测性能还需要通过更多临床试验进一步验证。
