National Institute of Immunohaematology (ICMR), Mumbai, India.
Int J Lab Hematol. 2010 Feb;32(1 Pt 1):e57-63. doi: 10.1111/j.1751-553X.2008.01096.x. Epub 2008 Aug 25.
Accurate estimation of hemoglobin (Hbs) A, Hb A(2), Hb F and abnormal Hb is required for diagnosis of hemoglobinopathies and genetic counseling. High pressure liquid chromatography (HPLC) is the most suitable approach available. But for 70% of the rural Indian population, HPLC analysis facilities are not available and screening would require transportation of samples to laboratories in bigger cities. We thus evaluated the feasibility of using a kit designed for measuring Hb A(1c) using capillary blood for collection and preservation of samples over a period of 15 days at different temperatures for screening for hemoglobinopathies. Capillary blood (5 microl) of 90 individuals was collected in the capillary collection system and run on the Variant Hemoglobin Testing System on days 1, 3, 5, 8, 12 and 15 after incubation at 4, 22, 37, 42 and 50 degrees C. The stability of different Hbs varied at different temperatures. The stability was maintained for 12 to 15 days by most of the samples up to 37 degrees C. Hb E was stable for 3 days up to at 37 degrees C and Hb D and Hb Q for 3 days up to 42 degrees C. This capillary blood collection system would have tremendous potential for sample collection and transportation under adverse climatic conditions for screening of hemoglobinopathies in remote areas in different countries.
准确估计血红蛋白（Hb）A、Hb A（2）、Hb F 和异常 Hb 对于诊断血红蛋白病和遗传咨询是必需的。高压液相色谱（HPLC）是目前最适用的方法。但对于 70%的印度农村人口来说，HPLC 分析设施不可用，筛查需要将样本运送到较大城市的实验室。因此，我们评估了使用设计用于测量 Hb A（1c）的试剂盒的可行性，该试剂盒使用毛细管血收集和保存样本，在不同温度下可保存 15 天，用于筛查血红蛋白病。在 4、22、37、42 和 50°C 孵育 1、3、5、8、12 和 15 天后，90 个人的毛细管血（5 微升）用毛细管收集系统收集，并在变异血红蛋白检测系统上运行。不同 Hb 在不同温度下的稳定性不同。大多数样本在 37°C 以下可稳定保存 12 至 15 天。Hb E 在 37°C 以下稳定 3 天，Hb D 和 Hb Q 在 42°C 以下稳定 3 天。这种毛细管血采集系统在不同国家的偏远地区，在恶劣的气候条件下，对于血红蛋白病的筛查，具有巨大的样本采集和运输潜力。
