非洲爪蟾蛋白酶体亚基XC3的推导一级结构及其mRNA在早期发育过程中的表达。

Deduced primary structure of a Xenopus proteasome subunit XC3 and expression of its mRNA during early development.

作者信息

Fujii G, Tashiro K, Emori Y, Saigo K, Tanaka K, Shiokawa K

机构信息

Laboratory of Molecular Embryology, Faculty of Science, University of Tokyo, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Aug 15;178(3):1233-9. doi: 10.1016/0006-291x(91)91025-8.

DOI:10.1016/0006-291x(91)91025-8

PMID:1872843

Abstract

Proteasome is a non-lysosomal proteinase complex ubiquitously distributed in eukaryotic cells. We isolated here the cDNA clone for one of the proteasome subunits (XC3) from Xenopus ovary cDNA libraries using rat RC3 cDNA as a prove. The cDNA is 885 bp long and encodes 234 amino acids. The deduced amino acid sequence is highly homologous (95.3%) to those of rat RC3 and human HC3 subunits. The mRNA for XC3 is one of the maternal mRNAs and detected at all the embryonic stages investigated, but its level changes in a characteristic way especially at the gastrula stage. We suggest that the highly conserved XC3 subunit plays an essential role in proteasome function and also that during Xenopus embryogenesis mRNA for XC3 subunit is replaced from maternal to newly-synthesized one probably around the gastrula stage.

摘要

蛋白酶体是一种非溶酶体蛋白酶复合体，广泛分布于真核细胞中。我们以大鼠RC3 cDNA为探针，从非洲爪蟾卵巢cDNA文库中分离出一种蛋白酶体亚基（XC3）的cDNA克隆。该cDNA长885 bp，编码234个氨基酸。推导的氨基酸序列与大鼠RC3和人HC3亚基的序列高度同源（95.3%）。XC3的mRNA是母源mRNA之一，在所研究的所有胚胎阶段均能检测到，但其水平以一种特殊的方式变化，尤其是在原肠胚阶段。我们认为，高度保守的XC3亚基在蛋白酶体功能中起重要作用，并且在非洲爪蟾胚胎发育过程中，XC3亚基的mRNA可能在原肠胚阶段左右从母源型被新合成型所取代。