Thomson S, Balson D F, Rivett A J
Department of Biochemistry, University of Leicester, UK.
FEBS Lett. 1993 May 10;322(2):135-8. doi: 10.1016/0014-5793(93)81553-c.
The primary structure of a new type of subunit (RN3) of rat proteasomes (multicatalytic proteinase complexes) has been determined from the nucleotide sequence of the cDNA. The cDNA encodes a protein of 232 amino acids but the directly determined N-terminal amino acid sequence suggests that the subunit is post-translationally processed to a M(r) = 24k form. Sequence alignments reveal a similarity of RN3 to other proteasome subunits. It can be designated a B-type proteasomal subunit but is not closely related to the beta subunit of the archaebacterial proteinase or to other members of the B group.
已从大鼠蛋白酶体（多催化蛋白酶复合物）新型亚基（RN3）的cDNA核苷酸序列确定了其一级结构。该cDNA编码一个含232个氨基酸的蛋白质，但直接测定的N端氨基酸序列表明该亚基经翻译后加工成M(r)=24k的形式。序列比对显示RN3与其他蛋白酶体亚基具有相似性。它可被指定为B型蛋白酶体亚基，但与古细菌蛋白酶的β亚基或B组的其他成员没有密切关系。
