Jiang Y, Tabak L A, Valente A J, Graves D T
Department of Oral Biology, Boston University Medical Center, MA 02118.
Biochem Biophys Res Commun. 1991 Aug 15;178(3):1400-4. doi: 10.1016/0006-291x(91)91049-i.
MCP-1 is the principle monocyte chemoattractant produced by tumor cells in vitro. We previously identified different isoforms of MCP-1. Here we report that two predominant isoforms of MCP-1 (13 kD and 9 kD) can be distinguished by lectin binding. The results indicate that the disaccharide galactose-beta 1-3D-N-acetyl galactosamine is present on the 13 kD MCP-1 isoform but not the 9 kD isoform. Glycosylation of the 13 kD MCP-1 isoform does not appear to significantly change its antigenic properties and also does not affect its capacity to induce monocyte migration.
单核细胞趋化蛋白-1（MCP-1）是肿瘤细胞在体外产生的主要单核细胞趋化因子。我们之前鉴定出了MCP-1的不同异构体。在此我们报告，MCP-1的两种主要异构体（13 kD和9 kD）可通过凝集素结合加以区分。结果表明，二糖半乳糖-β 1-3D-N-乙酰半乳糖胺存在于13 kD的MCP-1异构体上，而9 kD异构体上则没有。13 kD的MCP-1异构体的糖基化似乎并未显著改变其抗原特性，也不影响其诱导单核细胞迁移的能力。
