多巴胺-β-羟化酶抑制剂对嗜铬粒细胞膜摄取单胺的干扰作用。

Interference of inhibitors of dopamine-beta-hydroxylase with uptake of monoamines by chromaffin granular membranes.

作者信息

Schlichter D, Da Prada M, Pletscher A

出版信息

Eur J Pharmacol. 1975 Nov;34(1):223-7. doi: 10.1016/0014-2999(75)90243-5.

Abstract

Disulfiram and bis-(4-methyl-1-homopiperazinylthiocarbonyl)-disulphide (FLA 63) markedly inhibited the Mg2+/ATP-dependent uptake of various monoamines, e.g. dopamine (DA), by isolated membranes of bovine adrenal chromaffin granules. Both compounds affected DA-beta-hydroxylase (DBH) activity more markedly than the uptake of DA. Other inhibitors of DBH, e.g. fusaric acid and diethyldithiocarbaminate (DDC), did not interfere with DA uptake. Disulfiram and FLA 63, in contrast to fusaric acid and DDC, also caused a partial inhibition of Mg2+-dependent ATPase. It is concluded that the inhibition of monoamine uptake by disulfiram and FLA 63 is not related to their effect on DBH.

摘要

双硫仑和双 -（4 - 甲基 - 1 - 高哌嗪基硫代羰基） - 二硫化物（FLA 63）显著抑制了牛肾上腺嗜铬颗粒分离膜对各种单胺（如多巴胺，DA）的Mg2 + / ATP依赖性摄取。这两种化合物对多巴胺β - 羟化酶（DBH）活性的影响比对DA摄取的影响更显著。其他DBH抑制剂，如富马酸和二乙基二硫代氨基甲酸盐（DDC），并不干扰DA的摄取。与富马酸和DDC相反，双硫仑和FLA 63也会对Mg2 + 依赖性ATP酶产生部分抑制作用。得出的结论是，双硫仑和FLA 63对单胺摄取的抑制作用与其对DBH的作用无关。