Clezar C, Weber L I, Malaquias G S, Silva P O
Laboratório de Genética Molecular, CTTMar, Universidade do Vale do Itajaí, Itajaí, SC, Brasil.
Genet Mol Res. 2008 Jun 24;7(2):567-72. doi: 10.4238/vol7-2gmr459.
Here, we investigated the gene that encodes the main protein component of the byssus in Perna perna (Ppfp) using a consensus fluorescent probe for the Mytilus group and by polymerase chain reaction amplification using specific and random primers. A 524-bp fragment resulting from polymerase chain reaction amplification was found to be homologous to the fp-1 gene of Mytilus coruscus. This fragment was identified as the 3' end of the Ppfp-1 gene, which included the heptapeptide coding sequence for Lys-Pro-Ser-Tyr-Pro-Pro-Ter (probably the incomplete last tandem repeat unit of the giant exon), the stop codon and the polyadenylation signal.
