Department of Microbiology & Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA.
Department of Physics, East Carolina University, Greenville, NC, USA.
J Cell Mol Med. 2009 Aug;13(8B):1920-1932. doi: 10.1111/j.1582-4934.2008.00481.x. Epub 2008 Aug 21.
Kaposi's sarcoma-associated herpesvirus (KSHV) has both latent and lytic phases of replication. The molecular switch that triggers a reactivation is still unclear. Cells from the S phase of the cell cycle provide apt conditions for an active reactivation. In order to specifically delineate the Raman spectra of cells supporting KSHV reactivation, we followed a novel approach where cells were sorted based on the state of infection (latent versus lytic) by a flow cytometer and then analysed by the Raman tweezers. The Raman bands at 785, 813, 830, 1095 and 1128 cm(-1) are specifically altered in cells supporting KSHV reactivation. These five peaks make up the Raman fingerprint of cells supporting KSHV reactivation. The physiological relevance of the changes in these peaks with respect to KSHV reactivation is discussed in the following report.
卡波济肉瘤相关疱疹病毒（KSHV）具有潜伏和裂解复制阶段。触发重新激活的分子开关尚不清楚。细胞周期的 S 期为主动重新激活提供了合适的条件。为了专门描绘支持 KSHV 重新激活的细胞的拉曼光谱，我们采用了一种新方法，通过流式细胞仪根据感染状态（潜伏型与裂解型）对细胞进行分类，然后通过拉曼镊子进行分析。在支持 KSHV 重新激活的细胞中，785、813、830、1095 和 1128 cm(-1)处的拉曼带发生了特异性改变。这五个峰构成了支持 KSHV 重新激活的细胞的拉曼指纹。本文讨论了这些峰的变化与 KSHV 重新激活的生理相关性。
