Velleman S G, Li X, Coy C S, McFarland D C
Department of Animal Sciences, The Ohio State University/Ohio Agricultural Research and Development Center, Wooster 44691, USA.
Poult Sci. 2008 Sep;87(9):1834-40. doi: 10.3382/ps.2008-00029.
The membrane-associated heparan sulfate proteoglycan families, consisting of the syndecans and glypicans, are low-affinity receptors for fibroblast growth factor 2 (FGF2) that are essential in regulating the cellular response to FGF2. Fibroblast growth factor 2 is a potent stimulator of skeletal muscle cell proliferation and a strong inhibitor of differentiation. The regulation of the expression of the syndecans and glypicans will likely play a role in modulating the effects of FGF2 on cellular growth properties. In the present study, the effect of FGF2 on the expression of syndecan-4 and glypican-1 was measured by real-time PCR during turkey myogenic satellite cell proliferation and differentiation in vitro. Both syndecan-4 and glypican-1 transcription were influenced by the addition of exogenous FGF2. Syndecan-4 mRNA expression was reduced only during proliferation, whereas glypican-1 expression was reduced during both proliferation and differentiation. These results suggest that FGF2 growth factor signaling is, in part, regulated by an autoregulatory loop involving FGF2 regulation of syndecan-4 and glypican-1 expression and will affect the growth of skeletal muscle by modulating the proliferation and differentiation of satellite cells.
由syndecan蛋白聚糖和glypican蛋白聚糖组成的膜相关硫酸乙酰肝素蛋白聚糖家族，是成纤维细胞生长因子2（FGF2）的低亲和力受体，在调节细胞对FGF2的反应中至关重要。成纤维细胞生长因子2是骨骼肌细胞增殖的有效刺激剂，也是分化的强力抑制剂。syndecan蛋白聚糖和glypican蛋白聚糖表达的调节可能在调节FGF2对细胞生长特性的影响中发挥作用。在本研究中，通过实时PCR测定了FGF2对火鸡成肌卫星细胞体外增殖和分化过程中syndecan-4和glypican-1表达的影响。添加外源性FGF2会影响syndecan-4和glypican-1的转录。syndecan-4 mRNA表达仅在增殖过程中降低，而glypican-1表达在增殖和分化过程中均降低。这些结果表明，FGF2生长因子信号传导部分受一个自调节环调控，该自调节环涉及FGF2对syndecan-4和glypican-1表达的调节，并将通过调节卫星细胞的增殖和分化来影响骨骼肌的生长。
