Velleman Sandra G, Clark Daniel L, Tonniges Jeffrey R
Department of Animal Sciences, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, OH 44691, United States.
Department of Animal Sciences, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, OH 44691, United States.
Comp Biochem Physiol A Mol Integr Physiol. 2018 Sep;223:33-41. doi: 10.1016/j.cbpa.2018.05.014. Epub 2018 May 19.
Posthatch skeletal muscle growth requires myogenic satellite cells and the dynamic expression of cell membrane-associated proteins. The membrane associated heparan sulfate proteoglycans, syndecan-4 and glypican-1, link the satellite cell niche to the intracellular environment. Sydnecan-4 and glypican-1 are differentially expressed with age in turkey satellite cells and their over-expression impacts both satellite cell proliferation and differentiation, but their effect on satellite cells from lines with different growth potentials is not known. The objective of the current study was to determine if syndecan-4 and glypican-1 regulation of satellite cell proliferation and differentiation is affected by age and growth selection. Pectoralis major satellite cells isolated at 1 d, 7 and 16-wk of age from a Randombred Control 2 (RBC2) line and a 16-wk body weight (F) line selected from the RBC2 line turkeys were studied. Syndecan-4 and glypican-1 expression was knocked down in both lines. The F-line cells proliferated faster than RBC2 line cells regardless of age, while differentiation tended to be greater in RBC2 line cells than F-line cells at each age. Syndecan-4 knockdown decreased proliferation at 7- and 16-wk but not 1 d cells, and increased differentiation at 1 d and 7 wk but not 16 wk cells. Glypican-1 knockdown differentially affected proliferation depending on cell age, whereas differentiation was decreased for 7- and 16-wk but not 1 d cells. These data suggest syndecan-4 and glypican-1 differentially affected satellite cell function in an age-dependent manner, but had little impact on differences in proliferation and differentiation due to growth selection.
孵化后骨骼肌的生长需要生肌卫星细胞和细胞膜相关蛋白的动态表达。膜相关硫酸乙酰肝素蛋白聚糖、syndecan - 4和磷脂酰肌醇蛋白聚糖 - 1将卫星细胞生态位与细胞内环境联系起来。Syndecan - 4和磷脂酰肌醇蛋白聚糖 - 1在火鸡卫星细胞中的表达随年龄变化，它们的过表达会影响卫星细胞的增殖和分化，但它们对具有不同生长潜力品系的卫星细胞的影响尚不清楚。本研究的目的是确定syndecan - 4和磷脂酰肌醇蛋白聚糖 - 1对卫星细胞增殖和分化的调节是否受年龄和生长选择的影响。研究了从随机繁殖对照2（RBC2）品系以及从RBC2品系火鸡中选育出的16周龄体重（F）品系中，在1日龄、7周龄和16周龄时分离的胸大肌卫星细胞。在两个品系中均敲低了syndecan - 4和磷脂酰肌醇蛋白聚糖 - 1的表达。无论年龄如何，F品系细胞的增殖速度都比RBC2品系细胞快，而在每个年龄阶段，RBC2品系细胞的分化程度往往高于F品系细胞。敲低syndecan - 4会降低7周龄和16周龄细胞而非1日龄细胞的增殖，并增加1日龄和7周龄细胞而非16周龄细胞的分化。敲低磷脂酰肌醇蛋白聚糖 - 1对增殖的影响因细胞年龄而异，而7周龄和16周龄细胞而非1日龄细胞的分化则降低。这些数据表明，syndecan - 4和磷脂酰肌醇蛋白聚糖 - 1以年龄依赖性方式差异影响卫星细胞功能，但对生长选择导致的增殖和分化差异影响不大。
