Barlas Nurhayat, Aydoğan Müfide
Zoology Section, Department of Biology, Faculty of Science, University of Hacettepe, Beytepe, 06532 Ankara, Turkey.
Arch Toxicol. 2009 Apr;83(4):341-9. doi: 10.1007/s00204-008-0354-2. Epub 2008 Aug 27.
The present study was performed to investigate the potential toxic effects of prenatal exposure to 4-tert-octylphenol (OP) on liver, kidney, spleen, and hematologic parameters of male and female rats in adult life. The rats were treated with OP subcutaneously in utero at doses of control (vehicle, corn oil), 100 or 250 mg/kg per day. After birth, the rats were allowed to grow until adulthood and then liver, kidney and spleen were investigated histopathologically. Also the blood collected from rats were analyzed for any hematologic changes. The red blood cells of male and female rats were decreased in 250 mg/kg per day OP-treated group compared with the control group. micro-RBC of male rats in high dose treatment groups were increased significantly compared with the controls and 100 mg/kg per day treatment groups. micro-RBC of female rats in treatment groups were increased in a dose-dependent manner compared with the controls. In liver, kidney and spleen of male and female rats treated with OP, degeneration of hepatic parenchyma, tubular degeneration and hemorrhage were observed in histopathologic examination. The hemosiderin deposition in spleen of the high-dose group was increased in both male and female rats. In conclusion, findings of this study demonstrate that maternal administration of high doses of OP causes adverse effects on spleen and liver tissues of male and female offsprings at adulthood. Specifically, OP caused decreases in the number of red blood cells indicated by increased destruction in the spleen.
本研究旨在探讨产前暴露于4-叔辛基苯酚（OP）对成年雄性和雌性大鼠肝脏、肾脏、脾脏及血液学参数的潜在毒性作用。大鼠在子宫内通过皮下注射给予OP，剂量分别为对照（载体，玉米油）、100或250 mg/kg/天。出生后，让大鼠生长至成年，然后对肝脏、肾脏和脾脏进行组织病理学研究。同时，对采集的大鼠血液进行分析，以检测血液学变化。与对照组相比，每天250 mg/kg OP处理组的雄性和雌性大鼠红细胞数量减少。高剂量处理组雄性大鼠的微红细胞数量与对照组和每天100 mg/kg处理组相比显著增加。处理组雌性大鼠的微红细胞数量与对照组相比呈剂量依赖性增加。在接受OP处理的雄性和雌性大鼠的肝脏、肾脏和脾脏中，组织病理学检查观察到肝实质变性、肾小管变性和出血。高剂量组雄性和雌性大鼠脾脏中的含铁血黄素沉积均增加。总之，本研究结果表明，孕期给予高剂量OP会对成年雄性和雌性后代的脾脏和肝脏组织产生不良影响。具体而言，OP导致脾脏中红细胞破坏增加，红细胞数量减少。
