Sebai Hichem, Ben-Attia Mossadok, Sani Mamane, Aouani Ezzedine, Ghanem-Boughanmi Néziha
Département des Sciences de la Vie, Faculté des Sciences de Bizerte, UR Ethnobotanie & Stress Oxydant, 7021 Zarzouna, Tunisia.
Arch Toxicol. 2009 Apr;83(4):335-40. doi: 10.1007/s00204-008-0348-0. Epub 2008 Aug 27.
Lipopolysaccharide (LPS), a glycolipid component of the cell wall of gram-negative bacteria can elicit a systemic inflammatory process leading to septic shock and death. Acute phase response is characterized by fever, leucocytosis, thrombocytopenia, altered metabolic responses and redox balance by inducing excessive reactive oxygen species (ROS) generation. Resveratrol (trans-3,5,4' trihydroxystilbene) is a natural polyphenol exhibiting antioxidant and anti-inflammatory properties. We investigated the protective effect of resveratrol on endotoxemia-induced acute phase response in rats. When acutely administered by i.p. route, resveratrol (40 mg/kg b.w.) counteracted the effect of a single injection of LPS (4 mg/kg b.w.) which induced fever, a decrease in white blood cells (WBC) and platelets (PLT) counts. When i.p. administered during 7 days at 20 mg/kg per day (subacute treatment), resveratrol abrogated LPS-induced erythrocytes lipoperoxidation and catalase (CAT) activity depression to control levels. In the plasma compartment, LPS increased malondialdehyde (MDA) via nitric monoxide (NO) elevation and decreased iron level. All these deleterious LPS effects were reversed by a subacute resveratrol pre-treatment via a NO independent way. Resveratrol exhibited potent protective effect on LPS-induced acute phase response in rats.
脂多糖（LPS）是革兰氏阴性菌细胞壁的一种糖脂成分，可引发全身炎症反应，导致感染性休克和死亡。急性期反应的特征为发热、白细胞增多、血小板减少、代谢反应改变以及通过诱导过量活性氧（ROS）生成而导致氧化还原平衡改变。白藜芦醇（反式-3,5,4'-三羟基茋）是一种具有抗氧化和抗炎特性的天然多酚。我们研究了白藜芦醇对大鼠内毒素血症诱导的急性期反应的保护作用。经腹腔注射急性给药时，白藜芦醇（40毫克/千克体重）抵消了单次注射LPS（4毫克/千克体重）所产生的影响，LPS单次注射可引起发热、白细胞（WBC）和血小板（PLT）计数减少。当以每天20毫克/千克的剂量腹腔注射7天（亚急性治疗）时，白藜芦醇消除了LPS诱导的红细胞脂质过氧化作用，并将过氧化氢酶（CAT）活性降低恢复至对照水平。在血浆中，LPS通过一氧化氮（NO）升高增加了丙二醛（MDA）含量，并降低了铁水平。通过亚急性白藜芦醇预处理，所有这些LPS的有害作用均通过不依赖NO的方式得到逆转。白藜芦醇对大鼠LPS诱导的急性期反应表现出强大的保护作用。
