Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Departement des Sciences Biologiques, Faculte des Sciences, Tunis, Tunisie.
J Physiol Pharmacol. 2012 Jun;63(3):293-301.
Doxorubicin (Dox), a widely used antitumor anthracycline antibiotic, plays an undisputed key role in the treatment of many neoplasic diseases. In this study, the protective role of resveratrol against Dox-induced erythrocytes and plasma toxicity has been evaluated in rats. Animals were treated with resveratrol (25 mg/kg b.w.) by intraperitoneal injection during 8 days. At the 4(th) day of treatment, rats were intraperitoneally injected with a single dose of Dox (20 mg/kg b.w.). At the end of the treatment, blood samples were collected following standard procedure and processed for oxidative stress parameters (malondialdehyde (MDA), carbonyl protein, free iron, calcium and H(2)O(2) levels), transaminases and antioxidant enzymes as catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). Data showed that Dox drastically increased erythrocytes and plasma MDA, free iron, H(2)O(2) and carbonyl proteins but decreased calcium levels and also decreased erythrocytes CAT, POD and SOD activity. Besides, Dox decreased plasma CAT and SOD but unexpectedly increased POD activity. Dox also increased plasma ALT and AST levels and decreased them into erythrocytes. Co-treatment with resveratrol counteracted almost all Dox's effects. In conclusion, Dox induced a pro-oxidative stress into erythrocytes and resveratrol exerted real antioxidant properties which can be attributed, at least in part, to free iron and calcium modulation.
多柔比星（Dox）是一种广泛使用的抗肿瘤蒽环类抗生素，在治疗许多肿瘤疾病方面发挥着不可替代的关键作用。在这项研究中，我们评估了白藜芦醇对多柔比星诱导的红细胞和血浆毒性的保护作用。动物通过腹腔注射给予白藜芦醇（25mg/kg b.w.），连续 8 天。在第 4 天的治疗中，大鼠腹腔内注射单次剂量的多柔比星（20mg/kg b.w.）。在治疗结束时，按照标准程序采集血样，并处理用于评估氧化应激参数（丙二醛（MDA）、羰基蛋白、游离铁、钙和 H2O2 水平）、转氨酶和抗氧化酶（CAT、POD 和 SOD）。数据显示，多柔比星显著增加了红细胞和血浆中的 MDA、游离铁、H2O2 和羰基蛋白，但降低了钙水平，同时还降低了红细胞中的 CAT、POD 和 SOD 活性。此外，多柔比星降低了血浆中的 CAT 和 SOD，但出人意料地增加了 POD 活性。多柔比星还增加了血浆中的 ALT 和 AST 水平，并将其降低到红细胞中。白藜芦醇的共同治疗几乎抵消了多柔比星的所有作用。总之，多柔比星诱导了红细胞中的促氧化应激，而白藜芦醇发挥了真正的抗氧化特性，这至少部分归因于游离铁和钙的调节。
