子宫内膜异位症易感性与肿瘤坏死因子基因多态性的关联

[Association between susceptibility of endometriosis and the gene polymorphism of tumor necrosis factor].

作者信息

Lu Dong-hong, Lin Jun, Deng Lin

机构信息

Affiliated Obstetrical and Gynecological Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2008 Apr 15;88(15):1032-5.

PMID:18754435

Abstract

OBJECTIVE

To evaluate the association between the susceptibility of endometriosis (EMS) and the gene polymorphism of tumor necrosis factor (TNF) in Chinese Han population.

METHODS

Peripheral blood samples were collected from 76 EMS patients and 87 normal controls. PCR-RFLP was used to detect the single base change polymorphism in both TNFalpha gene and TNFbeta gene.

RESULTS

The allele frequencies of the TNFalpha polymorphism were 0.9474 and 0.9253 in the EMS patients and the control group respectively, and the TNFbeta polymorphism were 0.0526 and 0.0747 respectively. The allele frequencies of the TNFbeta1 polymorphism were 0.4605 and 0.5115 in the EMS patients and the control group, and the TNFbeta2 polymorphism were 0.5395 and 0.4885. No difference in the gene and genotype frequencies of TNFalpha and TNFbeta was noted in the EMS patients and normal controls (all P > 0.05).

CONCLUSION

TNF gene polymorphism has no effect on EMS in Chinese Han population. Ethnic difference may exist in TNF allele frequency.

摘要

目的

评估中国汉族人群中子宫内膜异位症（EMS）易感性与肿瘤坏死因子（TNF）基因多态性之间的关联。

方法

采集76例EMS患者和87例正常对照者的外周血样本。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测TNFα基因和TNFβ基因中的单碱基变化多态性。

结果

EMS患者组和对照组中，TNFα多态性的等位基因频率分别为0.9474和0.9253，TNFβ多态性的等位基因频率分别为0.0526和0.0747。EMS患者组和对照组中，TNFβ1多态性的等位基因频率分别为0.4605和0.5115，TNFβ2多态性的等位基因频率分别为0.5395和0.4885。EMS患者和正常对照者的TNFα和TNFβ基因及基因型频率均无差异（均P>0.05）。