Koros Christos, Boukouvalas George, Gerozissis Kyriaki, Kitraki Efthimia
Laboratory of Histology and Embryology, Medical School, University of Athens, Athens, Greece.
Nutrition. 2009 Jan;25(1):85-7. doi: 10.1016/j.nut.2008.06.033. Epub 2008 Aug 27.
The role of leptin receptors (Ob-Rs) within the hypothalamus in the control of energy expenditure has well been established. However, their role and regulation in other brain areas, including the cerebellum, is largely unexplored. In the present study we examined whether Ob-R levels in the rat cerebellum are influenced by a high-fat diet and if these changes are sexually divergent during adolescence.
The fat diet (45% energy from fat) was applied from weaning to puberty (postnatal days [P] 22-42), from weaning to adulthood (P22-90), and from puberty to adulthood (P42-90) in female and male Wistar rats. Ob-R levels were detected by western blotting and the data from pubertal and adult rats were analyzed by two-way analysis of variance for the effects of diet and sex.
The fat diet affected Ob-R long isoform levels in a sexually dimorphic manner. In the cerebellum of all fat-fed male groups, Ob-R levels were reduced compared with their chow-fed counterparts (P < 0.05). In contrast, in female rat Ob-R levels were reduced only in the adult P22-90 group.
Our data show for the first time that Ob-R levels in the rat cerebellum are subject to diet-induced alterations and that these changes are sexually dimorphic.
下丘脑内瘦素受体（Ob-Rs）在能量消耗控制中的作用已得到充分证实。然而，它们在包括小脑在内的其他脑区中的作用和调节在很大程度上尚未被探索。在本研究中，我们研究了大鼠小脑中Ob-R水平是否受高脂饮食影响，以及这些变化在青春期是否存在性别差异。
对雌性和雄性Wistar大鼠从断奶至青春期（出生后第[P]22 - 42天）、从断奶至成年期（P22 - 90天）以及从青春期至成年期（P42 - 90天）给予高脂饮食（脂肪提供45%的能量）。通过蛋白质免疫印迹法检测Ob-R水平，对青春期和成年大鼠的数据进行双因素方差分析，以分析饮食和性别的影响。
高脂饮食以性别二态性方式影响Ob-R长亚型水平。在所有高脂喂养的雄性组小脑中，Ob-R水平与其正常饮食喂养的对应组相比降低（P < 0.05）。相反，在雌性大鼠中，Ob-R水平仅在成年P22 - 90组降低。
我们的数据首次表明，大鼠小脑中的Ob-R水平会受到饮食诱导的改变影响，且这些变化具有性别二态性。
