Subash S, Subramanian P
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamil Nadu, India.
Singapore Med J. 2008 Aug;49(8):650-5.
Hyperammonaemia is a major contributing factor to neurological abnormalities observed in hepatic encephalopathy and in congenital defects of ammonia detoxication. Ammonia toxicity results in free radical generation that leads to oxidative stress and tissue damage. Morin is a bioflavonoid, a constituent of many herbs and fruits that are used as herbal medicines and also several biological activities. Our aim was to investigate the effect of morin on circulatory liver markers, lipid peroxidation and antioxidant status in ammonium chloride (AC)-induced hyperammonaemic rats.
Male albino Wistar rats weighing 180-200 g were used for the study. The hyperammonaemia was induced by interaperitonial injection of AC (100 mg/kg body weight). Rats were treated with morin (30 mg/kg body weight) via oral administration. Administration of morin in hyperammonaemic rats reduced the levels of ammonia and urea. The antioxidant property of morin was studied by assessing the activities of thiobarbituric acid reactive substances (TBARS), hydroperoxides (HP) and liver markers (alanine transaminase, aspartate transaminase and alkaline phosphatase) and the levels of glutathione peroxidase, superoxide dismutase, catalase, reduced glutathione, vitamins A, C and E in AC-treated rats.
Oxidative stress was effectively modulated by morin administration. Morin significantly improved the status of antioxidants and decreased the levels of ammonia, urea, TBARS, HP and liver markers enzymes, as compared to the AC-treated group.
The study offers evidence for the antihyperammonaemic, hepatoprotective and antioxidant effects of morin against oxidative stress induced by AC.
高氨血症是在肝性脑病和先天性氨解毒缺陷中观察到的神经功能异常的主要促成因素。氨毒性导致自由基生成，进而引发氧化应激和组织损伤。桑色素是一种生物类黄酮，是许多用作草药的草药和水果的成分，并且具有多种生物活性。我们的目的是研究桑色素对氯化铵（AC）诱导的高氨血症大鼠循环肝脏标志物、脂质过氧化和抗氧化状态的影响。
使用体重为180 - 200 g的雄性白化Wistar大鼠进行研究。通过腹腔注射AC（100 mg/kg体重）诱导高氨血症。通过口服给予大鼠桑色素（30 mg/kg体重）。给高氨血症大鼠施用桑色素可降低氨和尿素水平。通过评估硫代巴比妥酸反应性物质（TBARS）、氢过氧化物（HP）和肝脏标志物（丙氨酸转氨酶、天冬氨酸转氨酶和碱性磷酸酶）的活性以及AC处理大鼠中谷胱甘肽过氧化物酶、超氧化物歧化酶、过氧化氢酶、还原型谷胱甘肽、维生素A、C和E的水平，研究桑色素的抗氧化特性。
施用桑色素可有效调节氧化应激。与AC处理组相比，桑色素显著改善了抗氧化剂状态，并降低了氨、尿素、TBARS、HP和肝脏标志物酶的水平。
该研究为桑色素对AC诱导的氧化应激的抗高氨血症、肝脏保护和抗氧化作用提供了证据。
