Bruschi M, Hatchikian C E, Golovleva L A, Gall J L
J Bacteriol. 1977 Jan;129(1):30-8. doi: 10.1128/jb.129.1.30-38.1977.
Different electron carriers of the non-desulfoviridin-containing, sulfate-reducing bacterium Desulfovibrio desulfuricans (Norway strain) have been studied. Two nonheme iron proteins, ferredoxin and rubredoxin, have been purified. This ferredoxin contains four atoms of non-heme iron and acid-labile sulfur and six residues of cysteine per molecule. Its amino acid composition suggests that it is homologous with the other Desulfovibrio ferredoxins. The rubredoxin is also an acidic protein of 6,000 molecular weight and contains one atom of iron and four cysteine residues per molecule. The amino acid composition and molecular weight of the cytochrome c3 from D. desulfuricans (strain Norway 4) are reported. Its spectral properties are very similar to those of the other cytochromes c3 (molecular weight, 13,000) of Desulfovibrio and show that it contains four hemes per molecule. This cytochrome has a very low redox potential and acts as a carrier in the coupling of hydrogenase and thiosulfate reductase in extracts of Desulfovibrio gigas and Desulfovibrio desulfuricans (Norway strain) in contrast to D. gigas cytochrome c3 (molecular weight, 13,000). A comparison of the activities of the cytochrome c3 (molecular weight, 13,000) of D. gigas and that of D. desulfuricans in this reaction suggests that these homologous proteins can have different specificity in the electron transfer chain of these bacteria.
对不含脱硫绿素的硫酸盐还原菌脱硫脱硫弧菌（挪威菌株）的不同电子载体进行了研究。已纯化出两种非血红素铁蛋白，即铁氧化还原蛋白和红素氧还蛋白。这种铁氧化还原蛋白每分子含有四个非血红素铁原子和酸不稳定硫以及六个半胱氨酸残基。其氨基酸组成表明它与其他脱硫弧菌铁氧化还原蛋白同源。红素氧还蛋白也是一种分子量为6000的酸性蛋白，每分子含有一个铁原子和四个半胱氨酸残基。报道了脱硫脱硫弧菌（挪威4菌株）细胞色素c3的氨基酸组成和分子量。其光谱性质与脱硫弧菌的其他细胞色素c3（分子量13000）非常相似，表明它每分子含有四个血红素。与巨大脱硫弧菌细胞色素c3（分子量13000）不同，这种细胞色素具有非常低的氧化还原电位，并在巨大脱硫弧菌和脱硫脱硫弧菌（挪威菌株）提取物中作为氢化酶和硫代硫酸盐还原酶偶联中的载体。对巨大脱硫弧菌和脱硫脱硫弧菌细胞色素c3（分子量13000）在该反应中的活性比较表明，这些同源蛋白在这些细菌的电子传递链中可能具有不同的特异性。
