构巢曲霉中brlAbeta的可诱导RNA干扰
Inducible RNA Interference of brlAbeta in Aspergillus nidulans.
作者信息
Barton L M, Prade R A
机构信息
Department of Microbiology and Molecular Genetics, Oklahoma State University, 307 LSE, Stillwater, OK 74078, USA.
出版信息
Eukaryot Cell. 2008 Nov;7(11):2004-7. doi: 10.1128/EC.00142-08. Epub 2008 Aug 29.
Abstract
An inducible RNA interference (RNAi) construct composed of inverted repeating alcA promoters flanking the developmental regulatory gene brlAbeta was tested in Aspergillus nidulans. On inducing medium, the RNAi strains failed to sporulate and lacked brlAalpha and brlAbeta expression. RNAi was specific for brlAbeta, but not brlAalpha, silencing, indicating brlAalpha regulation by brlAbeta.
摘要
由位于发育调控基因brlAbeta两侧的反向重复alcA启动子组成的可诱导RNA干扰（RNAi）构建体在构巢曲霉中进行了测试。在诱导培养基上，RNAi菌株无法形成孢子，且缺乏brlAalpha和brlAbeta的表达。RNAi对brlAbeta具有特异性，但对brlAalpha没有沉默作用，这表明brlAalpha受brlAbeta调控。
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