小RNA的参与以及qde基因在粗糙脉孢菌基因沉默途径中的作用。
Involvement of small RNAs and role of the qde genes in the gene silencing pathway in Neurospora.
作者信息
Catalanotto Caterina, Azzalin Gianluca, Macino Giuseppe, Cogoni Carlo
机构信息
Dipartimento di Biotecnologie Cellulari ed Ematologia, Sezione di Genetica Molecolare, Università di Roma La Sapienza, 00161 Roma, Italy.
出版信息
Genes Dev. 2002 Apr 1;16(7):790-5. doi: 10.1101/gad.222402.
Abstract
Small RNA molecules have been found to be specifically associated with posttranscriptional gene silencing (PTGS) in both plants and animals. Here, we find that small sense and antisense RNAs are also involved in PTGS in Neurospora crassa. The accumulation of these RNA molecules depends on the presence of functional qde-1 and qde-3 genes previously shown to be essential for gene silencing, but does not depend on a functional qde-2, indicating that this gene is involved in a downstream step of the gene silencing pathway. Supporting this idea, a purified QDE2 protein complex was found to contain small RNA molecules, suggesting that QDE2 could be part of a small RNA-directed ribonuclease complex involved in sequence-specific mRNA degradation.
摘要
人们发现，小RNA分子在植物和动物中均与转录后基因沉默（PTGS）存在特异性关联。在此，我们发现小正义RNA和反义RNA也参与了粗糙脉孢菌中的PTGS。这些RNA分子的积累依赖于功能性qde-1和qde-3基因的存在，先前研究表明这两个基因对基因沉默至关重要，但不依赖于功能性qde-2，这表明该基因参与基因沉默途径的下游步骤。支持这一观点的是，纯化的QDE2蛋白复合物被发现含有小RNA分子，这表明QDE2可能是参与序列特异性mRNA降解的小RNA导向核糖核酸酶复合物的一部分。
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本文引用的文献
1
On the role of RNA amplification in dsRNA-triggered gene silencing.RNA扩增在双链RNA引发的基因沉默中的作用
Cell. 2001 Nov 16;107(4):465-76. doi: 10.1016/s0092-8674(01)00576-1.
2
RNAi as random degradative PCR: siRNA primers convert mRNA into dsRNAs that are degraded to generate new siRNAs.RNA干扰即随机降解性PCR：小干扰RNA引物将信使核糖核酸转化为双链核糖核酸，双链核糖核酸被降解以产生新的小干扰RNA。
Cell. 2001 Nov 2;107(3):297-307. doi: 10.1016/s0092-8674(01)00537-2.
3
An extensive class of small RNAs in Caenorhabditis elegans.秀丽隐杆线虫中一类广泛的小RNA。
Science. 2001 Oct 26;294(5543):862-4. doi: 10.1126/science.1065329.
4
An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans.一类在秀丽隐杆线虫中可能具有调控作用的丰富的微小RNA。
Science. 2001 Oct 26;294(5543):858-62. doi: 10.1126/science.1065062.
5
Identification of novel genes coding for small expressed RNAs.编码小表达RNA的新基因的鉴定。
Science. 2001 Oct 26;294(5543):853-8. doi: 10.1126/science.1064921.
6
RNA interference: listening to the sound of silence.RNA干扰：聆听沉默之声。
Nat Struct Biol. 2001 Sep;8(9):746-50. doi: 10.1038/nsb0901-746.
7
Argonaute2, a link between genetic and biochemical analyses of RNAi.AGO2蛋白，RNA干扰的遗传学与生物化学分析之间的联系。
Science. 2001 Aug 10;293(5532):1146-50. doi: 10.1126/science.1064023.
8
Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing.与RNA干扰相关的基因和机制调控着控制秀丽隐杆线虫发育时间的小时间RNA的表达。
Cell. 2001 Jul 13;106(1):23-34. doi: 10.1016/s0092-8674(01)00431-7.
9
Gene silencing as an adaptive defence against viruses.基因沉默作为一种针对病毒的适应性防御机制。
Nature. 2001 Jun 14;411(6839):834-42. doi: 10.1038/35081168.
10
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.21个核苷酸的RNA双链体在培养的哺乳动物细胞中介导RNA干扰。
Nature. 2001 May 24;411(6836):494-8. doi: 10.1038/35078107.
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