Schuler A, Spolarics Z, Lang C H, Bagby G J, Nelson S, Spitzer J J
Department of Physiology, Louisiana State University Medical Center, New Orleans 70112.
Life Sci. 1991;49(12):899-906. doi: 10.1016/0024-3205(91)90175-b.
Alterations of glucose metabolism were investigated for 6 hours following an intraarterial injection of murine recombinant granulocyte-monocyte colony-stimulating factor (GM-CSF) (30 micrograms/kg body weight). GM-CSF resulted in a transient elevation of plasma glucose. The rate of whole body glucose appearance, as measured by infusion of [6-3H] glucose, was increased by about 10% between 0.5 and 3 hours following GM-CSF injection. In vivo glucose utilization of individual tissues was investigated by the tracer 2-deoxyglucose technique. At 30 min, GM-CSF increased glucose utilization by 80-90% in liver and lung, and 50-60% in skin and spleen. At 3 and 6 hours, glucose utilization by these tissues returned toward control levels except for lung. There was a 40-50% increase in glucose utilization by skeletal muscle 30 min after GM-CSF which was sustained for 6 hours. Glucose utilization of testis, ileum and kidney did not change significantly. Plasma concentrations of insulin, glucagon and tumor necrosis factor were not altered in response to GM-CSF. These findings indicate that some of the acute metabolic effects of a short-term administration of GM-CSF are observed in macrophage-rich tissues, and suggest that GM-CSF may be involved in the metabolic upregulation of immunologically active tissues.
在动脉内注射小鼠重组粒细胞 - 单核细胞集落刺激因子（GM - CSF）（30微克/千克体重）后，对葡萄糖代谢变化进行了6小时的研究。GM - CSF导致血浆葡萄糖短暂升高。通过输注[6 - 3H]葡萄糖测量的全身葡萄糖出现率，在GM - CSF注射后0.5至3小时之间增加了约10%。采用示踪剂2 - 脱氧葡萄糖技术研究了各个组织的体内葡萄糖利用率。在30分钟时，GM - CSF使肝脏和肺中的葡萄糖利用率提高了80 - 90%，皮肤和脾脏中的葡萄糖利用率提高了50 - 60%。在3小时和6小时时，除了肺之外，这些组织的葡萄糖利用率恢复到对照水平。GM - CSF注射30分钟后，骨骼肌的葡萄糖利用率增加了40 - 50%，并持续6小时。睾丸、回肠和肾脏的葡萄糖利用率没有明显变化。GM - CSF注射后，血浆胰岛素、胰高血糖素和肿瘤坏死因子的浓度没有改变。这些发现表明，短期给予GM - CSF的一些急性代谢效应在富含巨噬细胞的组织中可以观察到，并且提示GM - CSF可能参与免疫活性组织的代谢上调。
